Rabbit anti-Human ATPase Inhibitory Factor 1 Polyclonal Antibody | anti-ATP5IF1 antibody

Anti-ATPase Inhibitory Factor 1 Antibody, Rabbit Polyclonal

Cat. Num. AAA254792

• Gene Names: ATPIF1; IP; ATPI; ATPIP
• Reactivity: Human
• Applications: Immunoprecipitation, Immunohistochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: ATPase Inhibitory Factor 1, Antibody; Anti-ATPase Inhibitory Factor 1 Antibody, Rabbit Polyclonal; ATPase Inhibitory Factor 1 Antibody, Rabbit PAb, Antigen Affinity Purified; ATPase inhibitory factor 1; Anti-ATPI Antibody; Anti-ATPIF1 Antibody; Anti-ATPIP Antibody; Anti-IP Antibody; anti-ATP5IF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human ATPase Inhibitory Factor 1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-ATP5IF1 antibody: IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human ATPase Inhibitory Factor 1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human ATPase Inhibitory Factor 1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Anti-ATPIF1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:12 kDaObserved band size:15 kDa(We are unsure as to the identity of these extra bands.))

IP (Immunoprecipitation)

(ATPIF1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate4 uL anti-ATPIF1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-ATPIF1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 16 kDaObserved band size: 16 kDa)

IHC (Immunohistochemistry)

(Immunochemical staining of human ATPIF1 in human thyroid cancer with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-ATP5IF1 antibody

Heme oxygenase-1 (HO-1) and ATPase inhibitory factor (ATPIF) 1 is often overexpressed in different types of cancer cells. To gain a physiological significance, we treated the synthetic flavonoids in colon cancer cells, HT29 and HCT116 cells and confirmed that overexpression of both HO-1 and ATPIF1 was critical for tumor cell death with an impaired mitochondrial energetics. It would provide a strategy for developing selective anti-tumor candidates. A transgene-based model of a heteroplasmic lethal mtDNA deletion (mtDNAdelta) in adult Drosophila muscle. Stimulation of autophagy, activation of the PINK1/parkin pathway or decreased levels of mitofusin result in a selective decrease in mtDNAdelta. Decreased levels of mitofusin and increased levels of ATPIF1, an inhibitor of ATP synthase reversal-dependent mitochondrial repolarization, result in a further decrease in mtDNAdelta levels. The loss of ATPIF1 strongly protects against antimycin-induced ETC dysfunction and cell death by allowing for the maintenance of mitochondrial membrane potential. Inhibition of ATPIF1 ameliorates complex III blockade in primary hepatocytes, a cell type afflicted in severe mitochondrial disease, that inhibition of ATPIF1 can ameliorate severe ETC dysfunction in mitochondrial pathology.

NCBI and Uniprot Product Information

• NCBI GI #: 12585262
• NCBI GeneID: 93974
• UniProt Accession #: Q9UII2; Q5JXL8; Q6IAQ7; Q9BSL9
• Molecular Weight: 106
• NCBI Official Full Name: ATPase inhibitor, mitochondrial
• NCBI Official Synonym Full Names: ATPase inhibitory factor 1
• NCBI Official Symbol: ATPIF1
• NCBI Official Synonym Symbols: IP; ATPI; ATPIP
• NCBI Protein Information: ATPase inhibitor, mitochondrial; IF1; ATPase inhibitor protein; inhibitor of F(1)F(o)-ATPase; ATP synthase inhibitor protein
• UniProt Protein Name: ATPase inhibitor, mitochondrial
• UniProt Gene Name: ATPIF1
• UniProt Synonym Gene Names: ATPI; IF(1); IF1
• UniProt Entry Name: ATIF1_HUMAN