<
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human trachea using ATPIF1 antibody at dilution of 1:100 (40x lens).)
Rabbit anti-Human, Mouse ATPIF1 Polyclonal Antibody | anti-ATPIF1 antibody
ATPIF1 Polyclonal Antibody
Gene Names
ATPIF1; IP; ATPI; ATPIP
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, ELISA
Purity
Affinity Purification
Synonyms
ATPIF1, Antibody; ATPIF1 Polyclonal Antibody; IP; ATPI; ATPIP; ATPIF1; F1; anti-ATPIF1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence Length
106
Applicable Applications for anti-ATPIF1 antibody
WB, IF,ICC, IP, ELISA.
Application Notes
WB 1:500 - 1:1000
IF/ICC 1:50 - 1:100
IP: 0.5ug-4ug antibody for 200 ug-400 ug extracts of whole cells.
IF/ICC 1:50 - 1:100
IP: 0.5ug-4ug antibody for 200 ug-400 ug extracts of whole cells.
Species
Human
Immunogen
Recombinant Protein
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 26-106 of human ATPIF1 (NP_057395.1).
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human trachea using ATPIF1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human trachea using ATPIF1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human liver cancer using ATPIF1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human liver cancer using ATPIF1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human stomach using ATPIF1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human stomach using ATPIF1 antibody at dilution of 1:100 (40x lens).)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300 ug extracts of K-562 cells using 3ug [KO Validated] ATPIF1 Rabbit pAb (AAA10772). Western blot was performed from theimmunoprecipitate using [KO Validated] ATPIF1 Rabbit pAb (AAA10772) at a dilution of 1:3000)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300 ug extracts of K-562 cells using 3ug [KO Validated] ATPIF1 Rabbit pAb (AAA10772). Western blot was performed from theimmunoprecipitate using [KO Validated] ATPIF1 Rabbit pAb (AAA10772) at a dilution of 1:3000)
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using [KO Validated] ATPIF1 Rabbit pAb (AAA10772) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining)
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using [KO Validated] ATPIF1 Rabbit pAb (AAA10772) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining)
WB (Western Blot)
(Western blot analysis of lysates from wild type(WT) and ATPIF1 knockout (KO) 293T(KO)cells, using [KO Validated] ATPIF1 Rabbit pAb (AAA10772) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (at 1:10000 dilution. Lysates/proteins: 25?g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 1s.)
WB (Western Blot)
(Western blot analysis of lysates from wild type(WT) and ATPIF1 knockout (KO) 293T(KO)cells, using [KO Validated] ATPIF1 Rabbit pAb (AAA10772) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (at 1:10000 dilution. Lysates/proteins: 25?g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 1s.)
Related Product Information for anti-ATPIF1 antibody
This gene encodes a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified.
Background: Enables several functions, including ATPase binding activity; angiostatin binding activity; and mitochondrial proton-transporting ATP synthase complex binding activity. Involved in several processes, including mitochondrial depolarization; negative regulation of ATPase activity; and regulation of protein targeting to mitochondrion. Located in cell surface and mitochondrion.
Background: Enables several functions, including ATPase binding activity; angiostatin binding activity; and mitochondrial proton-transporting ATP synthase complex binding activity. Involved in several processes, including mitochondrial depolarization; negative regulation of ATPase activity; and regulation of protein targeting to mitochondrion. Located in cell surface and mitochondrion.
Product Categories/Family for anti-ATPIF1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Observed MW: 12 kDa
Calculated MW: 12 KDa
Calculated MW: 12 KDa
NCBI Official Full Name
ATPase inhibitor, mitochondrial
NCBI Official Synonym Full Names
ATPase inhibitory factor 1
NCBI Official Symbol
ATPIF1
NCBI Official Synonym Symbols
IP; ATPI; ATPIP
NCBI Protein Information
ATPase inhibitor, mitochondrial; IF1; ATPase inhibitor protein; inhibitor of F(1)F(o)-ATPase; ATP synthase inhibitor protein
UniProt Protein Name
ATPase inhibitor, mitochondrial
UniProt Gene Name
ATPIF1
UniProt Synonym Gene Names
ATPI; IF(1); IF1
UniProt Entry Name
ATIF1_HUMAN
Similar Products
Product Notes
The ATPIF1 atpif1 (Catalog #AAA10772) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATPIF1 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ATPIF1 can be used in a range of immunoassay formats including, but not limited to, WB, IF,ICC, IP, ELISA. WB 1:500 - 1:1000 IF/ICC 1:50 - 1:100 IP: 0.5ug-4ug antibody for 200 ug-400 ug extracts of whole cells. Researchers should empirically determine the suitability of the ATPIF1 atpif1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATPIF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
+1 (619) 604-9440