IHC (Immunohiostchemistry)
(AAA322073 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit ATRIP Polyclonal Antibody | anti-ATRIP antibody
ATRIP (Phospho-Ser224) Antibody
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Synonyms
ATRIP, Antibody; ATRIP (Phospho-Ser224) Antibody; AGS1; ATIP; ATM and Rad3 related interacting protein; ATM and Rad3-related-interacting protein; ATR interacting protein; ATR-interacting protein; Atrip; ATRIP_HUMAN; DKFZp762J2115; FLJ12343; MGC20625; MGC21482; MGC26740; anti-ATRIP antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ATRIP (Phospho-Ser224) antibody detects endogenous levels of total ATRIP (Phospho-Ser224)
Purity/Purification
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
664
Applicable Applications for anti-ATRIP antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human ATRIP (Phospho-Ser224)
Subcellular Location
Nucleus. Redistributes to discrete nuclear foci upon DNA damage.
Tissue Specificity
Ubiquitous.
Predicted Cross Reactivity
Bovine, Sheep, Rabbit
Similarity
Bovine (100%), Sheep (86%), Rabbit (88%)
Conjugation
Unconjugated
Epitope
Phospho Ser224
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohiostchemistry)
(AAA322073 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA322073 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of ATRIP (Phospho-Ser224) expression in Human mammary cell lysate)
WB (Western Blot)
(Western blot analysis of ATRIP (Phospho-Ser224) expression in Human mammary cell lysate)
Related Product Information for anti-ATRIP antibody
Description: Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.
Subunit Structure: Interacts with ATR (By similarity). Heterodimer with ATR. The heterodimer binds the RPA complex and is then recruited to single-stranded DNA. Interacts with CEP164 (via N-terminus). Interacts with CINP.
Post-translational Modifications: Phosphorylated by ATR.
Similarity: The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage. Belongs to the ATRIP family.
Subunit Structure: Interacts with ATR (By similarity). Heterodimer with ATR. The heterodimer binds the RPA complex and is then recruited to single-stranded DNA. Interacts with CEP164 (via N-terminus). Interacts with CINP.
Post-translational Modifications: Phosphorylated by ATR.
Similarity: The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage. Belongs to the ATRIP family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 85 kDa
Predicted: 86 kDa
Predicted: 86 kDa
NCBI Official Full Name
ATR-interacting protein isoform 3
NCBI Official Synonym Full Names
ATR interacting protein
NCBI Official Symbol
ATRIP
NCBI Protein Information
ATR-interacting protein
UniProt Protein Name
ATR-interacting protein
UniProt Gene Name
ATRIP
UniProt Synonym Gene Names
AGS1
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Product Notes
The ATRIP atrip (Catalog #AAA322073) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATRIP (Phospho-Ser224) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATRIP can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ATRIP atrip for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATRIP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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