IHC (Immunohiostchemistry)
(AAA327856 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human, Mouse ATXN7 Polyclonal Antibody | anti-ATXN7 antibody
ATXN7 Antibody
Gene Names
ATXN7; SCA7; OPCA3; ADCAII
Reactivity
Human, Mouse
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ATXN7, Antibody; ATXN7 Antibody; ADCAII; ATXN 7; OPCA III; OPCA3; SCA 7; SCA7; Spinocerebellar Ataxia 7; Spinocerebellar ataxia type 7 protein; anti-ATXN7 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
ATXN7 antibody detects endogenous levels of total ATXN7
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
892
Applicable Applications for anti-ATXN7 antibody
ELISA, IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human ATXN7
Subcellular Location
Cytoplasmic (isoform b) and Nuclear (isoform a)
Tissue Specificity
Isoform a and isoform b are expressed in CNS, but isoform a is expressed predominantly in the peripherical tissues. Isoform b is also highly expressed in the frontal lobe, skeletal muscle and spinal cord and is expressed at a lower level in the lung, lymphoblast and intestine.
Predicted Cross Reactivity
Pig, Horse, Rabbit, Dog, Chicken
Similarity
Pig (91%), Horse (82%), Rabbit (82%), Dog (91%), Chicken (82%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohiostchemistry)
(AAA327856 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA327856 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot)
(Western blot analysis of ATXN7 using HuvEc whole cell lysates)
WB (Western Blot)
(Western blot analysis of ATXN7 using HuvEc whole cell lysates)
Related Product Information for anti-ATXN7 antibody
Function: Acts as component of the STAGA transcription coactivator-HAT complex. Mediates the interaction of STAGA complex with the CRX and is involved in CRX-dependent gene activation. Necessary for microtubule cytoskeleton stabilization.
Subunit Structure: Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, GCN5L2, TAF5L, TAF6L, SUPT7L, TADA3L, TAD1L, TAF10, TAF12, TRRAP, TAF9 and ATXN7. The STAGA core complex is associated with a subcomplex required for histone deubiquitination composed of ATXN7L3, ENY2 and USP22. Interacts with SORBS1, PSMC1 and CRX. Interacts with TRRAP, GCN5L2 and TAF10. Interacts with alpha tubulin.
Post-translational Modifications: Proteolytically cleaved. The cleavage may be involved in SCA7 degeneration: the isoform fragments may exert distinct toxic influences that could contribute to selective neurodegeneration. Sumoylation decreases the aggregation propensity and cellular toxicity of forms with an expanded poly-Gln region but has no effect on subcellular location or interaction with components of the STAGA complex.
Similarity: Belongs to the ataxin-7 family.
Subunit Structure: Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, GCN5L2, TAF5L, TAF6L, SUPT7L, TADA3L, TAD1L, TAF10, TAF12, TRRAP, TAF9 and ATXN7. The STAGA core complex is associated with a subcomplex required for histone deubiquitination composed of ATXN7L3, ENY2 and USP22. Interacts with SORBS1, PSMC1 and CRX. Interacts with TRRAP, GCN5L2 and TAF10. Interacts with alpha tubulin.
Post-translational Modifications: Proteolytically cleaved. The cleavage may be involved in SCA7 degeneration: the isoform fragments may exert distinct toxic influences that could contribute to selective neurodegeneration. Sumoylation decreases the aggregation propensity and cellular toxicity of forms with an expanded poly-Gln region but has no effect on subcellular location or interaction with components of the STAGA complex.
Similarity: Belongs to the ataxin-7 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 95 kDa
Predicted: 96 kDa
Predicted: 96 kDa
NCBI Official Full Name
ataxin-7 isoform a
NCBI Official Synonym Full Names
ataxin 7
NCBI Official Symbol
ATXN7
NCBI Official Synonym Symbols
SCA7; OPCA3; ADCAII
NCBI Protein Information
ataxin-7
UniProt Protein Name
Ataxin-7
UniProt Gene Name
ATXN7
UniProt Synonym Gene Names
SCA7
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Product Notes
The ATXN7 atxn7 (Catalog #AAA327856) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATXN7 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ATXN7 can be used in a range of immunoassay formats including, but not limited to, ELISA, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ATXN7 atxn7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATXN7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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