Rabbit anti-Human AUP1 Polyclonal Antibody | anti-AUP1 antibody

Anti-AUP1 Antibody Picoband

Cat. Num. AAA126540

• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay, Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: AUP1, Antibody; Anti-AUP1 Antibody Picoband; anti-AUP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (varies by lot)

• Applicable Applications for anti-AUP1 antibody: ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot)
• Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
• Immunogen: E Coli-derived human AUP1 recombinant protein (Position: A77-R399).
• Preparation and Storage: Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.

FCM/FACS (Flow Cytometry)

(Figure 2. Flow Cytometry analysis of U937 cells using anti-AUP1 antibody (AAA126540).Overlay histogram showing U937 cells stained with AAA126540 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AUP1 Antibody (AAA126540, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot)

(Figure 1. Western blot analysis of AUP1 using anti-AUP1 antibody (AAA126540).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human K562 whole cell lysates,Lane 2: human Jurkat whole cell lysates,Lane 3: human HEK293 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AUP1 antigen affinity purified polyclonal antibody (#AAA126540) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for AUP1 at approximately 42 kDa. The expected band size for AUP1 is at 42 kDa.)

Related Product Information for anti-AUP1 antibody

Ancient ubiquitous protein 1 is a protein that in humans is encoded by the AUP1 gene. The protein encoded this gene is involved in several pathways including quality control of misfolded proteins in the endoplasmic reticulum and lipid droplet accumulation. Lipid droplets are organelles in the cytoplasm that store neutral lipids such as cholesterol esters and trigylycerides to prevent the overabundance of free cholesterol and fatty acids in cells, but also to act as storage for other metabolic processes, such as membrane biogenesis. Reduced expression of this gene results in reduced lipid droplet clustering, a function that is dependent on ubiquitination of the protein. This protein contains multiple domains including a hydrophobic N-terminal domain, an acetyltranferase domain, a ubiquitin-binding CUE domain, and a UBE2B2-binding domain (G2BR). Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-AUP1 antibody

Primary Antibodies, Rabbit Polyclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 31712030
• NCBI GeneID: 550
• NCBI Accession #: NP_853553.1
• NCBI GenBank Nucleotide #: NM_181575.3
• UniProt Accession #: Q9Y679; Q68CX8
• Molecular Weight: 41,587 Da
• NCBI Official Full Name: ancient ubiquitous protein 1
• NCBI Official Synonym Full Names: ancient ubiquitous protein 1
• NCBI Official Symbol: AUP1
• NCBI Protein Information: ancient ubiquitous protein 1; OTTHUMP00000160424; OTTHUMP00000202724
• UniProt Protein Name: Ancient ubiquitous protein 1
• UniProt Gene Name: AUP1
• UniProt Entry Name: AUP1_HUMAN