WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells using B3GALT1 antibody.)
Rabbit anti-Human, Mouse B3GALT1 Polyclonal Antibody | anti-B3GALT1 antibody
B3GALT1 Antibody
Gene Names
B3GALT1; beta3Gal-T1
Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
B3GALT1, Antibody; B3GALT1 Antibody; 3-galactosyltransferase 1; 3-GalTase 1; B3GALT1; B3GT1_HUMAN; Beta 1, 3 galactosyltransferase 1; Beta 1, 3 GalTase 1; Beta 3 galt1; Beta-1; beta3Gal T1; Beta3Gal-T1; Beta3GalT1; UDP Gal:betaGlcNAc beta 1, 3 galactosyltransferase, polypeptide 1; UDP galactose:beta N acetyl glucosamine beta 1, 3 galactosyltransferase 1; UDP-galactose:beta-N-acetyl-glucosamine-beta-1; anti-B3GALT1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
B3GALT1 antibody detects endogenous levels of total B3GALT1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
326
Applicable Applications for anti-B3GALT1 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide
Subcellular Location
Golgi Apparatus Membrane.
Tissue Specificity
Detected in brain and colon mucosa and to a lesser extent in colon adenocarcinoma cells.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Similarity
Pig (100%), Bovine (100%), Horse (92%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (80%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells using B3GALT1 antibody.)
WB (Western Blot)
(Western blot analysis of extracts from HepG2 cells using B3GALT1 antibody.)
IF (Immunofluorescence)
(AAA324507 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(AAA324507 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-B3GALT1 antibody
Function: Beta-1, 3-galactosyltransferase that transfers galactose from UDP-alpha-D-galactose to substrates with a terminal beta-N-acetylglucosamine (beta-GlcNAc) residue. Involved in the biosynthesis of the carbohydrate moieties of glycolipids and glycoproteins. Inactive towards substrates with terminal alpha-N-acetylglucosamine (alpha-GlcNAc) or alpha-N-acetylgalactosamine (alpha-GalNAc) residues.
Similarity: Belongs to the glycosyltransferase 31 family.
Similarity: Belongs to the glycosyltransferase 31 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 36 kD
Predicted: 38 kDa
Predicted: 38 kDa
NCBI Official Full Name
beta-1,3-galactosyltransferase 1
NCBI Official Synonym Full Names
beta-1,3-galactosyltransferase 1
NCBI Official Symbol
B3GALT1
NCBI Official Synonym Symbols
beta3Gal-T1
NCBI Protein Information
beta-1,3-galactosyltransferase 1
UniProt Protein Name
Beta-1,3-galactosyltransferase 1
UniProt Gene Name
B3GALT1
UniProt Synonym Gene Names
Beta-1,3-GalTase 1; Beta3Gal-T1; Beta3GalT1
Similar Products
Product Notes
The B3GALT1 b3galt1 (Catalog #AAA324507) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The B3GALT1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's B3GALT1 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the B3GALT1 b3galt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B3GALT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.