FCM/FACS (Flow Cytometry)
(B4GalT1 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit B4GalT1 Polyclonal Antibody | anti-B4GALT1 antibody
B4GalT1 Antibody (C-term)
Gene Names
B4GALT1; GT1; GTB; CDG2D; GGTB2; B4GAL-T1; beta4Gal-T1
Reactivity
Human (Predicted Reactivity: Bovine, Mouse)
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
B4GalT1, Antibody; B4GalT1 Antibody (C-term); Beta-1,4-galactosyltransferase 1; Beta-1,4-GalTase 1; Beta4Gal-T1; b4Gal-T1; 241-; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1; Lactose synthase A protein; N-acetyllactosamine synthase; Nal synthase; Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase; Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase; Processed beta-1,4-galactosyltransferase 1; B4GALT1; GGTB2; anti-B4GALT1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Bovine, Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This B4GalT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 329-358 amino acids from the C-terminal region of human B4GalT1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
329-358
Sequence Length
385
Applicable Applications for anti-B4GALT1 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Clone Names
RB21685
Antigen Source
HUMAN
Function
The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
Cellular Location
Isoform Long: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein. Cell surface. Cell projection, filopodium {ECO:0000250|UniProtKB:P15535}. Note=Found in trans cisternae of Golgi but is mainly localized at the plasma membrane (PubMed:1714903). B4GALT1 cell surface expression is regulated by UBE2Q1 (By similarity). {ECO:0000250|UniProtKB:P15535, ECO:0000269|PubMed:1714903} Processed beta-1,4-galactosyltransferase 1: Secreted. Note=Soluble form found in body fluids.
Tissue Location
Ubiquitously expressed, but at very low levels in fetal and adult brain.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(B4GalT1 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(B4GalT1 Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of B4GalT1 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). B4GALT1 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of B4GalT1 Antibody (C-term) in HL-60 cell line lysates (35ug/lane). B4GALT1 (arrow) was detected using the purified Pab.)
Related Product Information for anti-B4GALT1 antibody
B4GalT1 is an enzyme that participates both in glycoconjugate and lactose biosynthesis. For the first activity, the enzyme adds galactose to N-acetylglucosamine residues that are either monosaccharides or the nonreducing ends of glycoprotein carbohydrate chains. The second activity is restricted to lactating mammary tissues where the enzyme forms a heterodimer with alpha-lactalbumin to catalyze UDP-galactose + D-glucose <=> UDP + lactose. The two enzymatic forms result from alternate transcription initiation sites and post-translational processing.
Product Categories/Family for anti-B4GALT1 antibody
References
Mengle-Gaw,L., et.al., Biochem. Biophys. Res. Commun. 176 (3), 1269-1276 (1991)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43920
NCBI Official Full Name
beta-1,4-galactosyltransferase 1
NCBI Official Synonym Full Names
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1
NCBI Official Symbol
B4GALT1
NCBI Official Synonym Symbols
GT1; GTB; CDG2D; GGTB2; B4GAL-T1; beta4Gal-T1
NCBI Protein Information
beta-1,4-galactosyltransferase 1
UniProt Protein Name
Beta-1,4-galactosyltransferase 1
UniProt Gene Name
B4GALT1
UniProt Synonym Gene Names
GGTB2; Beta-1,4-GalTase 1; Beta4Gal-T1; b4Gal-T1
UniProt Entry Name
B4GT1_HUMAN
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Product Notes
The B4GALT1 b4galt1 (Catalog #AAA284341) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 329-358. The B4GalT1 Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's B4GalT1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the B4GALT1 b4galt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B4GalT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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