IF (Immunofluorescence)
(Immunofluorescent analysis of B4GALT4 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Rabbit B4GALT4 Polyclonal Antibody | anti-B4GALT4 antibody
Anti-B4GALT4 Antibody
Gene Names
B4GALT4; B4Gal-T4; beta4Gal-T4
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Purified by Immunogen Affinity Chromatography
Synonyms
B4GALT4, Antibody; Anti-B4GALT4 Antibody; Beta-1 4-galactosyltransferase 4; Beta-1 4-GalTase 4; Beta4Gal-T4; b4Gal-T4; UDP-Gal:beta-GlcNAc beta-1 4-galactosyltransferase 4; UDP-galactose:beta-N-acetylglucosamine beta-1 4-galactosyltransferase 4; anti-B4GALT4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of B4GALT4 protein.
Purity/Purification
Purified by Immunogen Affinity Chromatography
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Applicable Applications for anti-B4GALT4 antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
Recombinant fusion protein of human B4GALT4. The exact sequence is proprietary.
Preparation and Storage
Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent analysis of B4GALT4 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
IF (Immunofluorescence)
(Immunofluorescent analysis of B4GALT4 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
WB (Western Blot)
(Western blot analysis of B4GALT4 expression in HT29 (A), mouse kidney (B), rat lung (C) whole cell lysates.)
WB (Western Blot)
(Western blot analysis of B4GALT4 expression in HT29 (A), mouse kidney (B), rat lung (C) whole cell lysates.)
Related Product Information for anti-B4GALT4 antibody
Rabbit polyclonal antibody to B4GALT4
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,041 Da
NCBI Official Full Name
beta-1,4-galactosyltransferase 4
NCBI Official Synonym Full Names
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4
NCBI Official Symbol
B4GALT4
NCBI Official Synonym Symbols
B4Gal-T4; beta4Gal-T4
NCBI Protein Information
beta-1,4-galactosyltransferase 4; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4; UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase 4; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4; beta-1,4-GalTase 4; beta-N-acetylglucosa
UniProt Protein Name
Beta-1,4-galactosyltransferase 4
UniProt Gene Name
B4GALT4
UniProt Synonym Gene Names
Beta-1,4-GalTase 4; Beta4Gal-T4; b4Gal-T4
UniProt Entry Name
B4GT4_HUMAN
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Product Notes
The B4GALT4 b4galt4 (Catalog #AAA264281) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-B4GALT4 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's B4GALT4 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the B4GALT4 b4galt4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B4GALT4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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