FCM/FACS (Flow Cytometry)
(B4GALT6 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit B4GALT6 Polyclonal Antibody | anti-B4GALT6 antibody
B4GALT6 Antibody (C-term)
Gene Names
B4GALT6; B4Gal-T6; beta4Gal-T6
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Western Blot, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
B4GALT6, Antibody; B4GALT6 Antibody (C-term); Beta-1,4-galactosyltransferase 6; Beta-1,4-GalTase 6; Beta4Gal-T6; b4Gal-T6; 241-; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 6; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 6; Glucosylceramide beta-1,4-galactosyltransferase; Lactosylceramide synthase; LacCer synthase; UDP-Gal:glucosylceramide beta-1,4-galactosyltransferase; B4GALT6; anti-B4GALT6 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This B4GALT6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 319-346 amino acids from the C-terminal region of human B4GALT6.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Positions
319-346
Sequence Length
382
Applicable Applications for anti-B4GALT6 antibody
WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
Cellular Location
Golgi apparatus, Golgi stack memrane; Single-pass type II membrane protein. Note= Trans cisternae of Golgi stack.
Tissue Location
High expression in brain and adrenal gland, lower in liver, lung, colon and peripheral white blood cells.
Antigen Region
319-346
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(B4GALT6 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(B4GALT6 Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(B4GALT6 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the B4GALT6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(B4GALT6 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the B4GALT6 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(B4GALT6 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the B4GALT6 antibody detected the B4GALT6 protein (arrow).)
WB (Western Blot)
(B4GALT6 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the B4GALT6 antibody detected the B4GALT6 protein (arrow).)
Related Product Information for anti-B4GALT6 antibody
Function Catalyzes the synthesis of lactosylceramide (LacCer) via the transfer of galactose from UDP-galactose to glucosylceramide (GlcCer) (PubMed:3099851, PubMed:1551920, PubMed:24498430). LacCer is the starting point in the biosynthesis of all gangliosides (membrane-bound glycosphingolipids) which play pivotal roles in the CNS including neuronal maturation and axonal and myelin formation (By similarity)
Product Categories/Family for anti-B4GALT6 antibody
References
Landers, J.E., et al. Proc. Natl. Acad. Sci. U.S.A. 106(22):9004-9009(2009) Gevaert, K., et al. Nat. Biotechnol. 21(5):566-569(2003) Fan, Y., et al. DNA Seq. 13(1):1-8(2002) Amado, M., et al. Biochim. Biophys. Acta 1473(1):35-53(1999) Takizawa, M., et al. Biochim. Biophys. Acta 1438(2):301-304(1999)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44914
NCBI Official Full Name
beta-1,4-galactosyltransferase 6
NCBI Official Synonym Full Names
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 6
NCBI Official Symbol
B4GALT6
NCBI Official Synonym Symbols
B4Gal-T6; beta4Gal-T6
NCBI Protein Information
beta-1,4-galactosyltransferase 6
UniProt Protein Name
Beta-1,4-galactosyltransferase 6
UniProt Gene Name
B4GALT6
UniProt Synonym Gene Names
Beta-1,4-GalTase 6; Beta4Gal-T6; b4Gal-T6; LacCer synthase
UniProt Entry Name
B4GT6_HUMAN
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Product Notes
The B4GALT6 b4galt6 (Catalog #AAA288174) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 319-346. The B4GALT6 Antibody (C-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's B4GALT6 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the B4GALT6 b4galt6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B4GALT6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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