Rabbit anti-Mouse B7-H2/ICOSLG Polyclonal Antibody | anti-B7-H2/ICOSLG antibody

Anti-B7-H2/ICOSLG Antibody, Rabbit Polyclonal

Cat. Num. AAA259125

• Reactivity: Mouse
• Applications: Immunoprecipitation, ELISA, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: B7-H2/ICOSLG, Antibody; Anti-B7-H2/ICOSLG Antibody, Rabbit Polyclonal; inducible T-cell co-stimulator ligand; Anti-B7-H2 Antibody; Anti-B7h Antibody; Anti-B7RP-1 Antibody; Anti-GI50 Antibody; Anti-GL50 Antibody; Anti-GL50-B Antibody; Anti-ICOS-L Antibody; Anti-Icoslg Antibody; Anti-LICOS Antibody; anti-B7-H2/ICOSLG antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse B7-H2/ICOSLG
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-B7-H2/ICOSLG antibody: IP (Immunoprecipitation), ELISA, WB (Western Blot)
• Immunogen: Recombinant Mouse ICOS ligand Protein
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse ICOS ligand (rM ICOS ligand). ICOS ligand specific IgG was purified by Mouse ICOS ligand affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

WB (Western Blot)

(Anti-ICOS Ligand/ICOSL rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse heart tissue lysateLane B: Mouse kidney tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:33 kDa)

IP (Immunoprecipitation)

(mB7H2 was immunoprecipitated using:Lane A:0.5 mg Mouse liver Whole Cell LysateLane B:0.5 mg Mouse kidney Whole Cell Lysate4 uL anti-mB7H2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-mB7H2 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 33 kDaObserved band size :36 kDa)

Related Product Information for anti-B7-H2/ICOSLG antibody

Inducible co-stimulator ligand (ICOSL), also known as B7-H2, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains and binds to ICOS on activated T cells, thus delivers a positive costimulatory signal for optimal T cell function. The structural features of ICOSL are crucial for its costimulatory function. The present study shows that ICOSL displays a marked oligomerization potential, resembling more like B7-1 than B7-2. B7-H2-dependent signaling may play an active role in a proliferative response rather than in cytokine and chemokine production. The CD28/B7 and ICOS/B7-H2 pathways are both critical for costimulating T cell immune responses. Deficiency in either pathway results in defective T cell activation, cytokine production, and germinal center formation.

References

Flesch IE. (2002) Inducible costimulator-ligand (ICOS-L). J Biol Regul Homeost Agents. 16(3): 217-9.
Kajiwara K, et al. (2009) Expression and function of the inducible costimulator ligand B7-H2 in human airway smooth muscle cells. Allergol Int. 58(4): 573-83.
Wong SC, et al. (2009) Functional hierarchy and relative contribution of the CD28/B7 and ICOS/B7-H2 costimulatory pathways to T cell-mediated delayed-type hypersensitivity. Cell Immunol. 256(1-2): 64-71.