Application Data
(BACH2 Antibody (C-term) (AAA285103) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negativecontrol cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human BACH2 Polyclonal Antibody | anti-BACH2 antibody
BACH2 Antibody (C-term)
Gene Names
BACH2; BTBD25
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
BACH2, Antibody; BACH2 Antibody (C-term); Transcription regulator protein BACH2; BTB and CNC homolog 2; BACH2; anti-BACH2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This BACH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 815-841 amino acids from the C-terminal region of human BACH2.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Applicable Applications for anti-BACH2 antibody
ELISA, WB (Western Blot), FCM/FACS (Flow Cytometry)
Antigen Region
815-841
Cellular Location
Cytoplasm. Nucleus {ECO:0000255|PROSITE- ProRule:PRU00978, ECO:0000269|PubMed:17018862, ECO:0000269|PubMed:28530713}. Note=Nucleocytoplasmic shuttling is controlled by phosphorylation.
Tissue Location
B-cell specific.
Function
Transcriptional regulator that acts as repressor or activator (By similarity). Binds to Maf recognition elements (MARE) (By similarity). Plays an important role in coordinating transcription activation and repression by MAFK (By similarity). Induces apoptosis in response to oxidative stress through repression of the antiapoptotic factor HMOX1. Positively regulates the nuclear import of actin (By similarity).
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application Data
(BACH2 Antibody (C-term) (AAA285103) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negativecontrol cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Application Data
(BACH2 Antibody (C-term) (AAA285103) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negativecontrol cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Application Data
(All lanes : Anti-BACH2 Antibody (C-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: Daudi whole cell lysate Lane 3: K562 whole cell lysate Lane 4: Ramos whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 93 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Application Data
(All lanes : Anti-BACH2 Antibody (C-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: Daudi whole cell lysate Lane 3: K562 whole cell lysate Lane 4: Ramos whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 93 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Application Data
(Overlay histogram showing Ramos cells stained with AAA285103 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA285103, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x106 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Application Data
(Overlay histogram showing Ramos cells stained with AAA285103 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA285103, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x106 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Product Categories/Family for anti-BACH2 antibody
References
Hinks, A., et al. Ann. Rheum. Dis. (2010) In press :
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010)
Pierce, B.L., et al. Hum. Hered. 69(3):193-201(2010)
Barrett, J.C., et al. Nat. Genet. 41(6):703-707(2009)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010)
Pierce, B.L., et al. Hum. Hered. 69(3):193-201(2010)
Barrett, J.C., et al. Nat. Genet. 41(6):703-707(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
transcription regulator protein BACH2
NCBI Official Synonym Full Names
BTB and CNC homology 1, basic leucine zipper transcription factor 2
NCBI Official Symbol
BACH2
NCBI Official Synonym Symbols
BTBD25
NCBI Protein Information
transcription regulator protein BACH2
UniProt Protein Name
Transcription regulator protein BACH2
UniProt Gene Name
BACH2
UniProt Entry Name
BACH2_HUMAN
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Product Notes
The BACH2 bach2 (Catalog #AAA285103) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BACH2 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BACH2 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the BACH2 bach2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BACH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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