WB (Western Blot)
(mouse BAD Antibody (S134) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the BAD antibody detected the BAD protein (arrow).)
Rabbit BAD Polyclonal Antibody | anti-Bad antibody
Mouse BAD Antibody (Center S134)
Gene Names
Bad; Bbc2; AI325008
Reactivity
Mouse; Predicted Reactivity: Rat
Applications
ELISA, Western Blot
Synonyms
BAD, Antibody; Mouse BAD Antibody (Center S134); Bcl2-associated agonist of cell death; BAD; Bcl-2-binding component 6; Bcl-xL/Bcl-2-associated death promoter; Bcl2 antagonist of cell death; Bad; Bbc6; anti-Bad antibody
Host
Rabbit
Reactivity
Mouse; Predicted Reactivity: Rat
Clonality
Polyclonal
Isotype
Ig
Specificity
This Mouse BAD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 112-141 amino acids from the Central region of mouse BAD.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Length
204
Applicable Applications for anti-Bad antibody
ELISA, WB (Western Blot)
Subtitle
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Cellular Location
Mitochondrion outer membrane. Cytoplasm. Note=Upon phosphorylation, locates to the cytoplasm.
Antigen Region
112-141 aa
Antigen Type
Synthetic Peptide
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(mouse BAD Antibody (S134) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the BAD antibody detected the BAD protein (arrow).)
WB (Western Blot)
(mouse BAD Antibody (S134) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the BAD antibody detected the BAD protein (arrow).)
WB (Western Blot)
(All lanes : Anti-mouse BAD Antibody at 1:1000 dilutionLane 1: A20 whole cell lysateLane 2: C2C12 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 22 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-mouse BAD Antibody at 1:1000 dilutionLane 1: A20 whole cell lysateLane 2: C2C12 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 22 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-Bad antibody
Description: BAD promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
Background: Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
Background: Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.
Product Categories/Family for anti-Bad antibody
References
Santidrian, A.F., et al. Blood 116 (16):3023-3032 (2010). Frenzel, A., et al. Blood 115 (5):995-1005 (2010). Quoyer, J., et al. J. Biol. Chem. 285 (3):1989-2002 (2010). Polzien, L., et al. J. Biol. Chem. 284 (41):28004-28020 (2009). Wu, X., et al. Diabetologia 52 (10):2130-2141 (2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22080 Da
NCBI Official Full Name
bcl2-associated agonist of cell death isoform 1
NCBI Official Synonym Full Names
BCL2-associated agonist of cell death
NCBI Official Symbol
Bad
NCBI Official Synonym Symbols
Bbc2; AI325008
NCBI Protein Information
bcl2-associated agonist of cell death
UniProt Protein Name
Bcl2-associated agonist of cell death
UniProt Gene Name
Bad
UniProt Synonym Gene Names
Bbc6; BAD
UniProt Entry Name
BAD_MOUSE
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Product Notes
The Bad bad (Catalog #AAA290680) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mouse BAD Antibody (Center S134) reacts with Mouse; Predicted Reactivity: Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BAD can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the Bad bad for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BAD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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