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IF (Immunofluorescence)
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Rabbit anti-Human, Mouse BAG1 Polyclonal Antibody | anti-BAG1 antibody

BAG1 Polyclonal Antibody

Gene Names
BAG1; HAP; BAG-1; RAP46
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Synonyms
BAG1, Antibody; BAG1 Polyclonal Antibody; Bag 1; BAG1; BAG1S; HAP; BAG1M; BAG1L specific; RAP46; anti-BAG1 antibody
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For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
345
Applicable Applications for anti-BAG1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:20 - 1:100
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human BAG1
Calculated Molecular Weight
39kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

IF (Immunofluorescence)

(Immunofluorescence analysis of U2OS cells using BAG1 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human esophagus using BAG1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human esophageal cancer using BAG1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human kidney injury using BAG1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human kidney cancer using BAG1 Antibody at dilution of 1:100 (40x lens).)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using BAG1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

Related Product Information for anti-BAG1 antibody
Bag1 belongs to the Bcl-2 associated athanogene (BAG) family of multifunctional proteins and was the first of six related proteins isolated from humans (1,2). This widely expressed protein interacts with a number of signaling molecules (including Bcl2, HGF receptor and Raf1) as it regulates signaling molecules in pathways involving cell survival, growth and differentiation. The most common role played by Bag1 protein is as an inhibitor of proteins favoring apoptosis (2-4). Bag1 also plays a role in Raf1 signaling and binds DNA as a transcription activator (4). Bag1 protein is a well-characterized inhibitor of its binding partner HSP70 (5). A conserved carboxy-terminal BAG domain within Bag1 interacts with the ATPase domain of HSP70 to negatively regulate heat shock protein chaperone activity (6,7). The multiple isoforms of Bag1 protein generated from a single transcript share a common ubiquitin homology domain and a carboxy-terminal Hsp70 binding region but differ in length and cellular localization. The 50 kDa long (Bag1-L) isoform also contains a nuclear localization signal and is often found in the nucleus where it activates transcription. The 46 kDa intermediate (Bag1-M) isoform is found mainly in the cytoplasm but can also translocate to the nucleus when associated with other proteins. The shorter 29-33 kDa isoforms (Bag1-S, Bag-1) isoforms are found primarily in the cytoplasm (8). High expression of the anti-apoptotic Bag1 protein correlates with increased survival in patients with particular forms of cancer, leading researchers to study possible therapeutic roles for Bag1 protein (9)
Product Categories/Family for anti-BAG1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
573
UniProt Accession #
Molecular Weight
345
NCBI Official Full Name
BAG family molecular chaperone regulator 1
NCBI Official Synonym Full Names
BCL2-associated athanogene
NCBI Official Symbol
BAG1
NCBI Official Synonym Symbols
HAP; BAG-1; RAP46
NCBI Protein Information
BAG family molecular chaperone regulator 1; Bcl-2-binding protein; receptor-associated protein, 46-KD; Bcl-2 associating athanogene-1 protein; glucocortoid receptor-associated protein RAP46
UniProt Protein Name
BAG family molecular chaperone regulator 1
UniProt Gene Name
BAG1
UniProt Synonym Gene Names
HAP; BAG-1
UniProt Entry Name
BAG1_HUMAN

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Product Notes

The BAG1 bag1 (Catalog #AAA10702) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BAG1 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BAG1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:20 - 1:100. Researchers should empirically determine the suitability of the BAG1 bag1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BAG1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.