Human Mannose Associated Serine Protease 2 ELISA Kit | MASP2 elisa kit
Human Mannose Associated Serine Protease 2 ELISA Kit
Gene Names
MASP2; sMAP; MAP19; MASP-2; MASP1P1
Reactivity
Human
Synonyms
Mannose Associated Serine Protease 2; N/A; Human Mannose Associated Serine Protease 2 ELISA Kit; MASP2 elisa kit
Reactivity
Human
Sequence Length
686
Samples
Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
7-1500ng/ml
Sensitivity
3.89ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Related Product Information for MASP2 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Mannose Associated Serine Protease 2 (also known as MASP2) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human MASP2 antibody. MASP2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human MASP2 Antibody is added and binds to MASP2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MASP2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human MASP2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human MASP2 antibody. MASP2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human MASP2 Antibody is added and binds to MASP2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated MASP2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human MASP2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mannan-binding lectin serine protease 2 isoform 1 preproprotein
NCBI Official Synonym Full Names
mannan binding lectin serine peptidase 2
NCBI Official Symbol
MASP2
NCBI Official Synonym Symbols
sMAP; MAP19; MASP-2; MASP1P1
NCBI Protein Information
mannan-binding lectin serine protease 2
UniProt Protein Name
Mannan-binding lectin serine protease 2
UniProt Gene Name
MASP2
UniProt Synonym Gene Names
MASP-2
UniProt Entry Name
MASP2_HUMAN