WB (Western Blot)
(Western blot analysis of extracts from 293, using BARD1 Antibody.)
Rabbit anti-Human BARD1 Polyclonal Antibody | anti-BARD1 antibody
BARD1 Antibody
Gene Names
DMC1; DMC1H; LIM15; dJ199H16.1
Reactivity
Human
Applications
ELISA, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
BARD1, Antibody; BARD1 Antibody; Disrupted meiotic cDNA 1 homolog; Disrupted meiotic cDNA 1, yeast, homolog of; dJ199H16.1; DMC 1; DMC1; DMC1 dosage suppressor of mck1 homolog; DMC1 dosage suppressor of mck1 homolog meiosis specific homologous recombination (yeast); DMC1 homologue; DMC1_HUMAN; DMC1H; DNA meiotic recombinase 1; HsLim15; LIM15; Meiotic recombination protein DMC1/LIM15 homolog; MGC150472; MGC150473; anti-BARD1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
BARD1 antibody detects endogenous levels of total BARD1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
285
Applicable Applications for anti-BARD1 antibody
ELISA, WB (Western Blot)
Immunogen
A synthesized peptide derived from human BARD1
Subcellular Location
Nucleus. Chromosome.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from 293, using BARD1 Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from 293, using BARD1 Antibody.)
WB (Western Blot)
(Western blot analysis on HeLa cell lysate using BARD1 Antibody, The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis on HeLa cell lysate using BARD1 Antibody, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-BARD1 antibody
Description: BARD1 the BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage. Colocalizes with BRCA1 into discrete subnuclear foci during S phase. Can translocate to the cytoplasm.
Function: May participate in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks.
Subunit Structure: Interacts with the MND1-PSMC3IP heterodimer (By similarity). Double stacked ring-shaped homooctamer. Interacts with BRCA2.
Similarity: Belongs to the RecA family. DMC1 subfamily.
Function: May participate in meiotic recombination, specifically in homologous strand assimilation, which is required for the resolution of meiotic double-strand breaks.
Subunit Structure: Interacts with the MND1-PSMC3IP heterodimer (By similarity). Double stacked ring-shaped homooctamer. Interacts with BRCA2.
Similarity: Belongs to the RecA family. DMC1 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 86 kDa
Predicted: 38 kDa
Predicted: 38 kDa
NCBI Official Full Name
meiotic recombination protein DMC1/LIM15 homolog isoform 2
NCBI Official Synonym Full Names
DNA meiotic recombinase 1
NCBI Official Symbol
DMC1
NCBI Official Synonym Symbols
DMC1H; LIM15; dJ199H16.1
NCBI Protein Information
meiotic recombination protein DMC1/LIM15 homolog
UniProt Protein Name
Meiotic recombination protein DMC1/LIM15 homolog
UniProt Gene Name
DMC1
UniProt Synonym Gene Names
DMC1H; LIM15
Similar Products
Product Notes
The BARD1 dmc1 (Catalog #AAA321002) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BARD1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BARD1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the BARD1 dmc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BARD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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