Rabbit Bassoon Polyclonal Antibody | anti-BSN antibody

Bassoon Antibody: ATTO 488

Cat. Num. AAA103563

• Gene Names: BSN; ZNF231
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Synonyms: Bassoon, Antibody; Bassoon Antibody: ATTO 488; BSN; ZNF231; Neuronal double zine finger protein; anti-BSN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 3926

• Applicable Applications for anti-BSN antibody: IHC (Immunohistochemistry), WB (Western Blot)
• Conjugate: ATTO 488
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

WB (Western Blot)

(Western blot analysis of Mouse, Rat brain cell lysates showing detection of Bassoon protein using Rabbit Anti-Bassoon Polyclonal Antibody (SPC-198). Primary Antibody: Rabbit Anti-Bassoon Polyclonal Antibody (SPC-198) at 1:1000.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis using Rabbit Anti-Bassoon Polyclonal Antibody (SPC-198). Tissue: Muscle. Species: Mouse. Primary Antibody: Rabbit Anti-Bassoon Polyclonal Antibody (SPC-198) at 1:400. Secondary Antibody: Alexa Fluor 488 Goat Anti-Rabbit. Counterstain: BTX (red). Localization: Selective staining of the NMJ.)

Related Product Information for anti-BSN antibody

Background Info: Detects ~420kDa, corresponding to the molecular mass of Bassoon. Multiple isoforms can be detected. No cross-reactivity to Piccolo.

Scientific Background: Bassoon (1) is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains. The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntington, abnormal amplification of this region can cause late-onset neurodegeneration. Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to co-localize with GABA (A) and glutamate (GluR1) receptors.

Product Categories/Family for anti-BSN antibody

Neuroscience

References

1. Anna Dondzillo et al., (2010) J. Comp Neur, 518(7): 1008-1029.

NCBI and Uniprot Product Information

• NCBI GI #: 91208420
• NCBI GeneID: 8927
• NCBI Accession #: NP_003449.2
• NCBI GenBank Nucleotide #: NM_003458.3
• UniProt Accession #: Q9UPA5; O43161; Q7LGH3
• Molecular Weight: 416,469 Da
• NCBI Official Full Name: protein bassoon
• NCBI Official Synonym Full Names: bassoon presynaptic cytomatrix protein
• NCBI Official Symbol: BSN
• NCBI Official Synonym Symbols: ZNF231
• NCBI Protein Information: protein bassoon; zinc finger protein 231; neuronal double zinc finger protein; bassoon (presynaptic cytomatrix protein)
• UniProt Protein Name: Protein bassoon
• UniProt Gene Name: BSN
• UniProt Synonym Gene Names: KIAA0434; ZNF231
• UniProt Entry Name: BSN_HUMAN