IHC (Immunohiostchemistry)
(Anti-BCAT1 antibody, AAA44954, IHC(P)IHC(P): Human Mammary Cancer Tissue)
Rabbit anti-Human BCAT1 Polyclonal Antibody | anti-BCAT1 antibody
Anti-BCAT1 antibody
Gene Names
BCAT1; BCT1; PP18; BCATC; ECA39; MECA39; PNAS121
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
BCAT1, Antibody; Anti-BCAT1 antibody; Branched-chain-amino-acid aminotransferase, cytosolic(BCAT(c)); branched chain amino-acid transaminase 1, cytosolic; BCAT antibody; BCAT(c) antibody; Bcat1 antibody; BCAT1_HUMAN antibody; BCT1 antibody; Branched chain amino acid aminotransferase, cytosolic antibody; branched chain amino-acid transaminase 1, cytosolic antibody; branched chain aminotransferase 1 antibody; branched chain aminotransferase 1, cytosolic antibody; Branched-chain-amino-acid aminotransferase antibody; cytosolic antibody; DKFZp686E12175 antibody; EC 2.6.1.42 antibody; ECA39 antibody; MECA39 antibody; placental protein 18 antibody; PNAS 121 antibody; PP18 antibody; Protein ECA39 antibody; anti-BCAT1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
386
Applicable Applications for anti-BCAT1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human BCAT1 (360-375aa KLASRILSKLTDIQYGR), different from the related mouse sequence by one amino acid, rat sequence by three amino acids.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(Anti-BCAT1 antibody, AAA44954, IHC(P)IHC(P): Human Mammary Cancer Tissue)
IHC (Immunohiostchemistry)
(Anti-BCAT1 antibody, AAA44954, IHC(P)IHC(P): Human Mammary Cancer Tissue)
WB (Western Blot)
(Anti-BCAT1 antibody, AAA44954, Western blottingAll lanes: Anti BCAT1 (AAA44954) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: JURKAT Whole Cell Lysate at 40ugPredicted bind size: 45KDObserved bind size: 45KD)
WB (Western Blot)
(Anti-BCAT1 antibody, AAA44954, Western blottingAll lanes: Anti BCAT1 (AAA44954) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: JURKAT Whole Cell Lysate at 40ugPredicted bind size: 45KDObserved bind size: 45KD)
Related Product Information for anti-BCAT1 antibody
Description: Rabbit IgG polyclonal antibody for Branched-chain-amino-acid aminotransferase, cytosolic(BCAT1) detection. Tested with WB, IHC-P in Human.
Background: BCAT1, Branched-chain Aminotransferase1, is also know as BCT1. The BCAT1 gene is highly expressed early in embryogenesis, and during organogenesis its expression is localized to the neural tube, the somites, and the mesonephric tubules. The gene is also expressed in several MYC-based tumors. The BCAT1 gene is mapped to chromosome 12. Lack of the enzyme BCT can cause auxotroph, a kind of auxotrophic mutant in Chinese-hamster ovary cells that lacks the ability to grow if alpha-ketoisovaleric acid, alpha-ketoisocaproic acid, and alpha-keto-beta-methylvaleric acid are substituted for valine, leucine, and isoleucine in the culture medium.
Background: BCAT1, Branched-chain Aminotransferase1, is also know as BCT1. The BCAT1 gene is highly expressed early in embryogenesis, and during organogenesis its expression is localized to the neural tube, the somites, and the mesonephric tubules. The gene is also expressed in several MYC-based tumors. The BCAT1 gene is mapped to chromosome 12. Lack of the enzyme BCT can cause auxotroph, a kind of auxotrophic mutant in Chinese-hamster ovary cells that lacks the ability to grow if alpha-ketoisovaleric acid, alpha-ketoisocaproic acid, and alpha-keto-beta-methylvaleric acid are substituted for valine, leucine, and isoleucine in the culture medium.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
44,131 Da
NCBI Official Full Name
Branched-chain-amino-acid aminotransferase, cytosolic
NCBI Official Synonym Full Names
branched chain amino-acid transaminase 1, cytosolic
NCBI Official Symbol
BCAT1
NCBI Official Synonym Symbols
BCT1; PP18; BCATC; ECA39; MECA39; PNAS121
NCBI Protein Information
branched-chain-amino-acid aminotransferase, cytosolic; placental protein 18; branched chain aminotransferase 1, cytosolic
UniProt Protein Name
Branched-chain-amino-acid aminotransferase, cytosolic
UniProt Gene Name
BCAT1
UniProt Synonym Gene Names
BCT1; ECA39; BCAT(c)
UniProt Entry Name
BCAT1_HUMAN
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Product Notes
The BCAT1 bcat1 (Catalog #AAA44954) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-BCAT1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BCAT1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the BCAT1 bcat1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BCAT1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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