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IF (Immunofluorescence)
(Confocal immunofluorescent analysis of BCKDHB Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
Rabbit anti-Human BCKDHB Polyclonal Antibody | anti-BCKDHB antibody
BCKDHB Antibody (N-term)
Gene Names
BCKDHB; E1B; dJ279A18.1
Reactivity
Human
Applications
Immunofluorescence, Flow Cytometry, Functional Assay, Western Blot, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
BCKDHB, Antibody; BCKDHB Antibody (N-term); 2-oxoisovalerate dehydrogenase subunit beta; mitochondrial; Branched-chain alpha-keto acid dehydrogenase E1 component beta chain; BCKDE1B; BCKDH E1-beta; BCKDHB; anti-BCKDHB antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This BCKDHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-70 amino acids from the N-terminal region of human BCKDHB.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.45 (varies by lot)
Sequence Positions
41-70
Sequence Length
218
Applicable Applications for anti-BCKDHB antibody
IF (Immunofluorescence), FCM/FACS (Flow Cytometry), WB (Western Blot), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of BCKDHB Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of BCKDHB Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
FCM/FACS (Flow Cytometry)
(BCKDHB Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(BCKDHB Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(Western blot analysis of BCKDHB (arrow) using rabbit polyclonal BCKDHB Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BCKDHB gene.)
WB (Western Blot)
(Western blot analysis of BCKDHB (arrow) using rabbit polyclonal BCKDHB Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the BCKDHB gene.)
IHC (Immunohiostchemistry)
(BCKDHB Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BCKDHB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(BCKDHB Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the BCKDHB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(BCKDHB Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the BCKDHB antibody detected the BCKDHB protein (arrow).)
WB (Western Blot)
(BCKDHB Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the BCKDHB antibody detected the BCKDHB protein (arrow).)
Related Product Information for anti-BCKDHB antibody
Branched-chain keto acid dehydrogenase is a multienzyme
complex associated with the inner membrane of mitochondria, and
functions in the catabolism of branched-chain amino acids. The
complex consists of multiple copies of 3 components: branched-chain
alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2)
and lipoamide dehydrogenase (E3). This gene encodes the E1 beta
subunit, and mutations therein have been associated with maple
syrup urine disease (MSUD), type 1B, a disease characterized by a
maple syrup odor to the urine in addition to mental and physical
retardation, and feeding problems.
complex associated with the inner membrane of mitochondria, and
functions in the catabolism of branched-chain amino acids. The
complex consists of multiple copies of 3 components: branched-chain
alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2)
and lipoamide dehydrogenase (E3). This gene encodes the E1 beta
subunit, and mutations therein have been associated with maple
syrup urine disease (MSUD), type 1B, a disease characterized by a
maple syrup odor to the urine in addition to mental and physical
retardation, and feeding problems.
Product Categories/Family for anti-BCKDHB antibody
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Gorzelany, K., et al. Turk. J. Pediatr. 51(2):97-102(2009)
Quental, S., et al. Mol. Genet. Metab. 94(2):148-156(2008)
Kang, H., et al. Fertil. Steril. 89(3):728-731(2008)
Flaschker, N., et al. J. Inherit. Metab. Dis. 30(6):903-909(2007)
Gorzelany, K., et al. Turk. J. Pediatr. 51(2):97-102(2009)
Quental, S., et al. Mol. Genet. Metab. 94(2):148-156(2008)
Kang, H., et al. Fertil. Steril. 89(3):728-731(2008)
Flaschker, N., et al. J. Inherit. Metab. Dis. 30(6):903-909(2007)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43122
NCBI Official Full Name
2-oxoisovalerate dehydrogenase subunit beta, mitochondrial
NCBI Official Synonym Full Names
branched chain keto acid dehydrogenase E1, beta polypeptide
NCBI Official Symbol
BCKDHB
NCBI Official Synonym Symbols
E1B; dJ279A18.1
NCBI Protein Information
2-oxoisovalerate dehydrogenase subunit beta, mitochondrial
UniProt Protein Name
2-oxoisovalerate dehydrogenase subunit beta, mitochondrial
UniProt Gene Name
BCKDHB
UniProt Synonym Gene Names
BCKDE1B; BCKDH E1-beta
UniProt Entry Name
ODBB_HUMAN
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Product Notes
The BCKDHB bckdhb (Catalog #AAA290133) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 41-70. The BCKDHB Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BCKDHB can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), FCM/FACS (Flow Cytometry), WB (Western Blot), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the BCKDHB bckdhb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BCKDHB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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