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IP (Immunoprecipitation)
(Detection of human Bcl-2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-Bcl-2 antibody AAA212258 used for IP at 6 ug/mg lysate. Bcl-2 was also immunoprecipitated by rabbit anti-Bcl-2 antibody BL13035, which recognizes an upstream epitope. For blotting immunoprecipitated Bcl-2, AAA212258 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human Bcl-2 Polyclonal Antibody | anti-Bcl-2 antibody
Rabbit anti-Bcl-2 Antibody, Affinity Purified
Gene Names
BCL2; Bcl-2; PPP1R50
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
Bcl-2, Antibody; Rabbit anti-Bcl-2 Antibody, Affinity Purified; Bcl-2; B-cell CLL/lymphoma 2; apoptosis regulator Bcl-2; protein phosphatase 1, regulatory subunit 50; PPP1R50; Apoptosis regulator Bcl-2; BCL2, apoptosis regulator; anti-Bcl-2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-Bcl-2 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen
Between 50 and 100
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human Bcl-2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-Bcl-2 antibody AAA212258 used for IP at 6 ug/mg lysate. Bcl-2 was also immunoprecipitated by rabbit anti-Bcl-2 antibody BL13035, which recognizes an upstream epitope. For blotting immunoprecipitated Bcl-2, AAA212258 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human Bcl-2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-Bcl-2 antibody AAA212258 used for IP at 6 ug/mg lysate. Bcl-2 was also immunoprecipitated by rabbit anti-Bcl-2 antibody BL13035, which recognizes an upstream epitope. For blotting immunoprecipitated Bcl-2, AAA212258 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IHC (Immunohistochemisry)
(Detection of human Bcl-2 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Bcl-2 (Cat. No. AAA212258 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemisry)
(Detection of human Bcl-2 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-Bcl-2 (Cat. No. AAA212258 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of human Bcl-2 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Bcl-2 (Cat. No. AAA212258 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of human Bcl-2 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Bcl-2 (Cat. No. AAA212258 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
FCM/FACS (Flow Cytometry)
(Detection of human Bcl-2 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-Bcl-2 polyclonal antibody (AAA212258) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
FCM/FACS (Flow Cytometry)
(Detection of human Bcl-2 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-Bcl-2 polyclonal antibody (AAA212258) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Related Product Information for anti-Bcl-2 antibody
B-cell CLL/lymphoma 2 (Bcl-2) is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of Bcl-2, such as in the case of translocation of Bcl-2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma [taken from NCBI Entrez Gene (Gene ID: 596)].
Product Categories/Family for anti-Bcl-2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,337 Da
NCBI Official Full Name
apoptosis regulator Bcl-2 beta isoform
NCBI Official Synonym Full Names
B-cell CLL/lymphoma 2
NCBI Official Symbol
BCL2
NCBI Official Synonym Symbols
Bcl-2; PPP1R50
NCBI Protein Information
apoptosis regulator Bcl-2
UniProt Protein Name
Apoptosis regulator Bcl-2
UniProt Gene Name
BCL2
UniProt Entry Name
BCL2_HUMAN
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Product Notes
The Bcl-2 bcl2 (Catalog #AAA212258) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Bcl-2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Bcl-2 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the Bcl-2 bcl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bcl-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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