Rabbit BCL-2 BINDING COMPONENT 3 Polyclonal Antibody | anti-BCL-2 antibody

RABBIT ANTI BCL-2 BINDING COMPONENT 3

Cat. Num. AAA49961

• Gene Names: BBC3; JFY1; PUMA; JFY-1
• Reactivity: Mouse (Expected from Sequence)
• Applications: Western Blot, Immunohistochemistry
• Synonyms: BCL-2 BINDING COMPONENT 3, Antibody; RABBIT ANTI BCL-2 BINDING COMPONENT 3; anti-BCL-2 antibody

• Host: Rabbit
• Reactivity: Mouse (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises the p53 - upregulated modulator of apoptosis (PUMA), also known as Bcl-2 binding component 3. Bcl-2 binding component 3 is a pro-apoptotic member of the BCL-2 family. ; ; Bcl-2 binding component 3 is localised to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 261

• Applicable Applications for anti-BCL-2 antibody: WB (Western Blot), IHC (Immunohistochemistry)
• Perservative Stabilisers: 0.02% Sodium Azide
• Immunogen: Synthetic peptide ARARQEGSSPEPVEG corresponding to the N-terminus of human Bcl-2 binding component 3.; Histology Positive Control Tissue
• Antiserum Preparation: Antisera to human Bcl-2 binding component 3 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Application Data

(Western blot analysis of whole cell lysate from K562 cells probed with Rabbit anti Human bcl-2 binding component 3 (AAA49961))

Application Data

(Staining of human breast carcinoma. Formalin fixed paraffin processed tissue with Rabbit anti Human PUMA (NT) at 10 ug/ml (AAA49961))

Related Product Information for anti-BCL-2 antibody

MBS221697 recognises the p53 - upregulated modulator of apoptosis (PUMA), also known as Bcl-2 binding component 3. Bcl-2 binding component 3 is a pro-apoptotic member of the BCL-2 family. Bcl-2 binding component 3 is localised to the mitochondria, binds BCL-2 and induces cytochrome C release and apoptosis in response to p53.

NCBI and Uniprot Product Information

• NCBI GI #: 187829730
• NCBI GeneID: 27113
• NCBI Accession #: NP_001120712.1
• NCBI GenBank Nucleotide #: NM_001127240.2
• UniProt Accession #: Q9BXH1; O00171; Q96PG9; B9EGI3; Q96PG8
• Molecular Weight: 10,326 Da
• NCBI Official Full Name: bcl-2-binding component 3 isoform 1
• NCBI Official Synonym Full Names: BCL2 binding component 3
• NCBI Official Symbol: BBC3
• NCBI Official Synonym Symbols: JFY1; PUMA; JFY-1
• NCBI Protein Information: bcl-2-binding component 3; p53 up-regulated modulator of apoptosis
• UniProt Protein Name: Bcl-2-binding component 3
• UniProt Gene Name: BBC3
• UniProt Synonym Gene Names: PUMA
• UniProt Entry Name: BBC3_HUMAN