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IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Rabbit anti-Human Bcl-G BH3 Domain Polyclonal Antibody | anti-BCL2L14 antibody
Bcl-G BH3 Domain Antibody
Gene Names
BCL2L14; BCLG
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Bcl-G BH3 Domain, Antibody; Bcl-G BH3 Domain Antibody; Apoptosis facilitator Bcl-2-like protein 14; Bcl2-L-14; Apoptosis regulator Bcl-G; BCL2L14; BCLG; anti-BCL2L14 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Bcl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 194-229 amino acids from human Bcl.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
194-229
Sequence Length
252
Applicable Applications for anti-BCL2L14 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(Cell lysates were subjected to Western blotting to determine the expression of Bcl-gS protein. The levels of alpha-tubulin were determined to assure equal loading.)
WB (Western Blot)
(Cell lysates were subjected to Western blotting to determine the expression of Bcl-gS protein. The levels of alpha-tubulin were determined to assure equal loading.)
WB (Western Blot)
(Western blot analysis of anti-Bcl-G BH3 Domain Pab in K562 cell line lysates (35ug/lane). Bcl-G BH3 Domain (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-Bcl-G BH3 Domain Pab in K562 cell line lysates (35ug/lane). Bcl-G BH3 Domain (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Anti-Bcl-G-BH3 Antibodyat 1:2000 dilution + human lung lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 37 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-Bcl-G-BH3 Antibodyat 1:2000 dilution + human lung lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 37 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-BCL2L14 antibody
Bcl-G belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Overexpression of Bcl-G has been shown to induce apoptosis in cells.
Product Categories/Family for anti-BCL2L14 antibody
References
Ozalp, S.S., et al., Eur J Gynaecol Oncol 23(5):419-422 (2002).
Guo, B., et al., J. Biol. Chem. 276(4):2780-2785 (2001).
Guo, B., et al., J. Biol. Chem. 276(4):2780-2785 (2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36598
NCBI Official Full Name
apoptosis facilitator Bcl-2-like protein 14 isoform 2
NCBI Official Synonym Full Names
BCL2-like 14 (apoptosis facilitator)
NCBI Official Symbol
BCL2L14
NCBI Official Synonym Symbols
BCLG
NCBI Protein Information
apoptosis facilitator Bcl-2-like protein 14
UniProt Protein Name
Apoptosis facilitator Bcl-2-like protein 14
UniProt Gene Name
BCL2L14
UniProt Synonym Gene Names
BCLG; Bcl2-L-14
UniProt Entry Name
B2L14_HUMAN
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Product Notes
The BCL2L14 bcl2l14 (Catalog #AAA283668) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 194-229. The Bcl-G BH3 Domain Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Bcl-G BH3 Domain can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the BCL2L14 bcl2l14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bcl-G BH3 Domain, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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