WB (Western Blot)
(Detection of human Bcl11b by western blot. Samples: Whole cell lysate (5 ug) from Hep-G2, MCF-7, Jurkat, RKO, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Bcl11b antibody (AAA210918 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Rabbit anti-Human Bcl11b Polyclonal Antibody | anti-Bcl11b antibody
Rabbit anti-Bcl11b Antibody, Affinity Purified
Gene Names
BCL11B; ATL1; RIT1; CTIP2; CTIP-2; ZNF856B; ATL1-beta; ATL1-alpha; ATL1-delta; ATL1-gamma; hRIT1-alpha
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Bcl11b, Antibody; Rabbit anti-Bcl11b Antibody, Affinity Purified; hRIT1-alpha; ZNF856B; zinc finger protein hRit1 alpha; RIT1; radiation-induced tumor suppressor gene 1 protein; IDDFSTA; hRit1; CTIP-2; CTIP2; COUP-TF-interacting protein 2; BCL11B/TRDC fusion; ATL1-gamma; IMD49; BCL-11B; ATL1-alpha; ATL1-beta; ATL1-delta; B-cell CLL/lymphoma 11B; ATL1; B-cell CLL/lymphoma 11B (zinc finger protein); B-cell CLL/lymphoma 11B/T-cell receptor delta constant region fusion protein; B-cell lymphoma/leukaemia 11B; B-cell lymphoma/leukemia 11B; B cell CLL/lymphoma 11B; anti-Bcl11b antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-Bcl11b antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human Bcl11b by western blot. Samples: Whole cell lysate (5 ug) from Hep-G2, MCF-7, Jurkat, RKO, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Bcl11b antibody (AAA210918 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot)
(Detection of human Bcl11b by western blot. Samples: Whole cell lysate (5 ug) from Hep-G2, MCF-7, Jurkat, RKO, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Bcl11b antibody (AAA210918 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human Bcl11b by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-Bcl11b antibody (AAA210918 lot 3) used for IP at 6 ug per reaction. Bcl11b was also immunoprecipitated by a previous lot of this antibody (AAA210918 lot 2) and a second antibody against a different epitope of Bcl11b . For blotting immunoprecipitated Bcl11b, AAA210918 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human Bcl11b by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 10% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-Bcl11b antibody (AAA210918 lot 3) used for IP at 6 ug per reaction. Bcl11b was also immunoprecipitated by a previous lot of this antibody (AAA210918 lot 2) and a second antibody against a different epitope of Bcl11b . For blotting immunoprecipitated Bcl11b, AAA210918 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Related Product Information for anti-Bcl11b antibody
Bcl11b is a zinc-finger transcription factor that is homologous to the proto-oncogene Bcl11a. Bcl11b functions in T-cell development, and as a tumor suppressor, plays a role in T-cell lymphoma pathogenesis. Bcl11b interacts with ARP1, SIRT1, and TFCOUP1 to modulate transcription.
Product Categories/Family for anti-Bcl11b antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
88,476 Da
NCBI Official Full Name
B-cell lymphoma/leukemia 11B isoform 2
NCBI Official Synonym Full Names
B-cell CLL/lymphoma 11B (zinc finger protein)
NCBI Official Symbol
BCL11B
NCBI Official Synonym Symbols
ATL1; RIT1; CTIP2; CTIP-2; ZNF856B; ATL1-beta; ATL1-alpha; ATL1-delta; ATL1-gamma; hRIT1-alpha
NCBI Protein Information
B-cell lymphoma/leukemia 11B; B-cell CLL/lymphoma 11B/T-cell receptor delta constant region fusion protein; B-cell lymphoma/leukaemia 11B; BCL-11B; BCL11B/TRDC fusion; COUP-TF-interacting protein 2; hRit1; radiation-induced tumor suppressor gene 1 protein
UniProt Protein Name
B-cell lymphoma/leukemia 11B
UniProt Gene Name
BCL11B
UniProt Synonym Gene Names
CTIP2; RIT1; BCL-11B; hRit1
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Product Notes
The Bcl11b bcl11b (Catalog #AAA210918) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Bcl11b Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Bcl11b can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the Bcl11b bcl11b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bcl11b, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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