WB (Western Blot)
(All lanes : Anti-BCL2L10 Antibody (Center) at 1:2000 dilutionLane 1: Human kidney lysateLane 2: Human liver lysateLane 3: Human lung lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 22 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human BCL2L10 Polyclonal Antibody | anti-BCL2L10 antibody
BCL2L10 Antibody (Center)
Gene Names
BCL2L10; Boo; Diva; BCL-B; bcl2-L-10
Reactivity
Human
Applications
ELISA, Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Purified through a protein A column, followed by peptide affinity purification.
Synonyms
BCL2L10, Antibody; BCL2L10 Antibody (Center); Purified Rabbit Polyclonal Antibody (Pab); Bcl-2-like protein 10; Bcl2-L-10; Anti-apoptotic protein NrH; Apoptosis regulator Bcl-B; BCL2L10; BCLB; anti-BCL2L10 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Ig
Specificity
This BCL2L10 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 89-123 amino acids from the Central region of human BCL2L10.
Purity/Purification
Purified through a protein A column, followed by peptide affinity purification.
Form/Format
Supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Length
241
Applicable Applications for anti-BCL2L10 antibody
ELISA, WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Antigen Type
Synthesized Peptide
Antigen Source
Human
Tissue Location
Widely expressed in adult tissues. Preferentially expressed in lung, liver and kidney
Cellular Location
Mitochondrion. Nucleus membrane
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks.
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(All lanes : Anti-BCL2L10 Antibody (Center) at 1:2000 dilutionLane 1: Human kidney lysateLane 2: Human liver lysateLane 3: Human lung lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 22 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-BCL2L10 Antibody (Center) at 1:2000 dilutionLane 1: Human kidney lysateLane 2: Human liver lysateLane 3: Human lung lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 22 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
IF (Immunofluorescence)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with AP22305c at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).)
IF (Immunofluorescence)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with AP22305c at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).)
Related Product Information for anti-BCL2L10 antibody
Promotes cell survival. Suppresses apoptosis induced by BAX but not BAK.
Product Categories/Family for anti-BCL2L10 antibody
References
Zhang H.,et al.Hum. Mol. Genet. 10:2329-2339(2001). Ke N.,et al.J. Biol. Chem. 276:12481-12484(2001). Aouacheria A.,et al.Oncogene 20:5846-5855(2001). Zody M.C.,et al.Nature 440:671-675(2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21973
NCBI Official Full Name
bcl-2-like protein 10 isoform 1
NCBI Official Synonym Full Names
BCL2 like 10
NCBI Official Symbol
BCL2L10
NCBI Official Synonym Symbols
Boo; Diva; BCL-B; bcl2-L-10
NCBI Protein Information
bcl-2-like protein 10
UniProt Protein Name
Bcl-2-like protein 10
UniProt Gene Name
BCL2L10
UniProt Synonym Gene Names
BCLB; Bcl2-L-10
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Product Notes
The BCL2L10 bcl2l10 (Catalog #AAA290891) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BCL2L10 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BCL2L10 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the BCL2L10 bcl2l10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BCL2L10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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