WB (Western Blot)
(Detection of human BCR by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-BCR antibody AAA211740 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. BCR was also immunoprecipitated by rabbit anti-BCR antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
Rabbit anti-Human BCR Polyclonal Antibody | anti-BCR antibody
Rabbit anti-BCR Antibody, Affinity Purified
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
BCR, Antibody; Rabbit anti-BCR Antibody, Affinity Purified; D22S11; PHL; BCR/FGFR1 chimera protein; FGFR1/BCR chimera protein; renal carcinoma antigen NY-REN-26; D22S662; breakpoint cluster region protein; BCR1; ALL; breakpoint cluster region; CML; Breakpoint cluster region protein; BCR, RhoGEF and GTPase activating protein; anti-BCR antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-BCR antibody
FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse
Immunogen
Between 1221 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human BCR by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-BCR antibody AAA211740 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. BCR was also immunoprecipitated by rabbit anti-BCR antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
WB (Western Blot)
(Detection of human BCR by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-BCR antibody AAA211740 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. BCR was also immunoprecipitated by rabbit anti-BCR antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
ICC (Immunocytochemistry)
(Detection of human BCR by immunocytochemistry. Sample: FFPE section of human K-562 cells (contains the chromosomal translocation, t(9:22) that creates the BCR/ABL fusion gene). Antibody: Affinity purified rabbit anti-BCR (Cat. No. AAA211740) used at a dilution of 1:1,000 (1ug/ml). Detection: Green-fluorescent goat anti-rabbit IgG-Alexa Fluor 488 used at a dilution of 1:1,000.)
ICC (Immunocytochemistry)
(Detection of human BCR by immunocytochemistry. Sample: FFPE section of human K-562 cells (contains the chromosomal translocation, t(9:22) that creates the BCR/ABL fusion gene). Antibody: Affinity purified rabbit anti-BCR (Cat. No. AAA211740) used at a dilution of 1:1,000 (1ug/ml). Detection: Green-fluorescent goat anti-rabbit IgG-Alexa Fluor 488 used at a dilution of 1:1,000.)
FCM/FACS (Flow Cytometry)
(Detection of BCR. 1 x 10^6 K-562 cells were fixed, permeabilized, and stained with 0.25 ug anti-BCR AAA211740in a 150 ul reaction. Isotype anti-KLH control (red), no antibody (blue) and anti-BCR AAA211740 (black).)
FCM/FACS (Flow Cytometry)
(Detection of BCR. 1 x 10^6 K-562 cells were fixed, permeabilized, and stained with 0.25 ug anti-BCR AAA211740in a 150 ul reaction. Isotype anti-KLH control (red), no antibody (blue) and anti-BCR AAA211740 (black).)
Related Product Information for anti-BCR antibody
BCR (breakpoint cluster region) was identified as part of a fusion protein that results from a reciprocal translocation with the ABL gene between chromosomes 22 and 9. The BCR-ABL fusion is the cause of chronic myeloid leukemia (CML). The BCR protein contains a C2 domain, a DBL-homology domain, a PH domain, and a Rho-GAP domain and functions as a GTPase-activating protein for RAC1 and CDC42.
Product Categories/Family for anti-BCR antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The BCR bcr (Catalog #AAA211740) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-BCR Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BCR can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ICC (Immunocytochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the BCR bcr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BCR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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