IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Rabbit Beclin 1 Polyclonal Antibody | anti-BECN1 antibody
Beclin 1 Antibody
Gene Names
BECN1; ATG6; VPS30; beclin1
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Chicken)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Beclin 1, Antibody; Beclin 1 Antibody; Beclin-1; Coiled-coil myosin-like BCL2-interacting protein; Protein GT197; BECN1; GT197; anti-BECN1 antibody
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Chicken)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Beclin 1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 181-210 amino acids from human Beclin 1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
181-210
Sequence Length
450
Applicable Applications for anti-BECN1 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(The anti-BECN1 Pab is used in Western blot to detect BECN1 in mouse liver tissue lysate. BECN1(arrow) was detected using the purified Pab.)
WB (Western Blot)
(The anti-BECN1 Pab is used in Western blot to detect BECN1 in mouse liver tissue lysate. BECN1(arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-hBECN1-Q196 Pab in 293 cell line lysates transiently transfected with the BECN1 gene (2ug/lane). hBECN1-Q196(arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of anti-hBECN1-Q196 Pab in 293 cell line lysates transiently transfected with the BECN1 gene (2ug/lane). hBECN1-Q196(arrow) was detected using the purified Pab.)
Related Product Information for anti-BECN1 antibody
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Beclin 1 plays a role in two fundamentally important cell biological pathways: autophagy and apoptosis. Beclin 1 is thought to function as a VPS and autophagy protein as part of a complex with Class III PI3 kinase, Vps34.
Product Categories/Family for anti-BECN1 antibody
References
Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005) Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005) Greenberg JT. Dev Cell. 8(6):799-801. (2005) Levine B. Cell. 120(2):159-62. (2005) Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004) Liang,X.H., et al. J. Virol. 72 (11), 8586-8596 (1998) Aita V.M., et al. Genomics 59:59-65(1999).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51896
NCBI Official Full Name
beclin-1
NCBI Official Synonym Full Names
beclin 1, autophagy related
NCBI Official Symbol
BECN1
NCBI Official Synonym Symbols
ATG6; VPS30; beclin1
NCBI Protein Information
beclin-1
UniProt Protein Name
Beclin-1
UniProt Gene Name
BECN1
UniProt Synonym Gene Names
GT197
UniProt Entry Name
BECN1_HUMAN
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Product Notes
The BECN1 becn1 (Catalog #AAA288444) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 181-210. The Beclin 1 Antibody reacts with Human, mouse (Predicted Reactivity: Bovine, Chicken) and may cross-react with other species as described in the data sheet. AAA Biotech's Beclin 1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the BECN1 becn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Beclin 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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