Application Data
(Formalin fixed, paraffin embedded rat brain stained with Rabbit anti Human beclin-1 (AAA49911) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))
Rabbit anti-Mouse, Rat BECLIN-1 Polyclonal Antibody | anti-Becn1 antibody
RABBIT ANTI BECLIN-1 (N-TERMINAL)
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Synonyms
BECLIN-1, Antibody; RABBIT ANTI BECLIN-1 (N-TERMINAL); anti-Becn1 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
This item recognises an epitope within the N-terminal region of Beclin-1, a 52kDa coiled-coil protein which shares structural homology with the yeast autophagy gene apg6/vps30.
The ability of cells to undergo bulk degradation of endogenous proteins through an autophagosomic-lysosomal pathway, termed autophagy or Type II cell death, is important in the control of cell growth and development. Studies have identified Beclin-1 as a Bcl-2 interacting mammalian gene which not only mediates autophagy, but also functions as a tumour suppressor, being expressed at reduced levels in human breast carcinoma, and may also play a role in defense against viral infection. An increasing number of studies have revealed an interaction between proteins which induce autophagy and those involved in apoptosis and in some instances apoptosis may be dependant upon or delayed by autophagy.
The ability of cells to undergo bulk degradation of endogenous proteins through an autophagosomic-lysosomal pathway, termed autophagy or Type II cell death, is important in the control of cell growth and development. Studies have identified Beclin-1 as a Bcl-2 interacting mammalian gene which not only mediates autophagy, but also functions as a tumour suppressor, being expressed at reduced levels in human breast carcinoma, and may also play a role in defense against viral infection. An increasing number of studies have revealed an interaction between proteins which induce autophagy and those involved in apoptosis and in some instances apoptosis may be dependant upon or delayed by autophagy.
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Sequence Length
448
Applicable Applications for anti-Becn1 antibody
WB (Western Blot), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry)
Perservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A 17 amino acid peptide located near human Beclin-1 amino-terminus.
Buffer Solution
Buffer Solution
Antiserum Preparation
Antisera to human Beclin-1 (NT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Application Data
(Formalin fixed, paraffin embedded rat brain stained with Rabbit anti Human beclin-1 (AAA49911) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))
Application Data
(Formalin fixed, paraffin embedded rat brain stained with Rabbit anti Human beclin-1 (AAA49911) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))
Application Data
(Western blot analysis of whole cell lysate from HEK293 human eptihelial kidney cells probed with Rabbit anti Human beclin-1 (AAA49911) at 0.5(A), 1(B) and 2(C) ug/ml)
Application Data
(Western blot analysis of whole cell lysate from HEK293 human eptihelial kidney cells probed with Rabbit anti Human beclin-1 (AAA49911) at 0.5(A), 1(B) and 2(C) ug/ml)
Related Product Information for anti-Becn1 antibody
MBS221318 recognises an epitope within the N-terminal region of Beclin-1, a 52kDa coiled-coil protein which shares structural homology with the yeast autophagy gene apg6/vps30. The ability of cells to undergo bulk degradation of endogenous proteins through an autophagosomic-lysosomal pathway, termed autophagy or Type II cell death, is important in the control of cell growth and development. Studies have identified Beclin-1 as a Bcl-2 interacting mammalian gene which not only mediates autophagy, but also functions as a tumour suppressor, being expressed at reduced levels in human breast carcinoma, and may also play a role in defense against viral infection. An increasing number of studies have revealed an interaction between proteins which induce autophagy and those involved in apoptosis and in some instances apoptosis may be dependant upon or delayed by autophagy.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,557 Da
NCBI Official Full Name
beclin-1
NCBI Official Synonym Full Names
beclin 1, autophagy related
NCBI Official Symbol
Becn1
NCBI Protein Information
beclin-1; beclin 1 (coiled-coil, myosin-like BCL2-interacting protein); coiled-coil myosin-like BCL2-interacting protein
UniProt Protein Name
Beclin-1
UniProt Gene Name
Becn1
UniProt Entry Name
BECN1_RAT
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Product Notes
The Becn1 becn1 (Catalog #AAA49911) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RABBIT ANTI BECLIN-1 (N-TERMINAL) reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BECLIN-1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the Becn1 becn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BECLIN-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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