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IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded Rat heart using beta actin Polycloanl Antibody at dilution of 1:200)
Rabbit beta ACTIN Polyclonal Antibody | anti-ACTB antibody
beta ACTIN Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purification
Synonyms
beta ACTIN, Antibody; beta ACTIN Polyclonal Antibody; A26C1A; A26C1B; ACTB; Actin beta; Actin cytoplasmic 1; Actin; Cytoplasmic 1; N-terminally processed; Actx; B actin; Beta cytoskeletal actin; Beta-actin; BRWS1; E430023M04Rik; MGC128179; PS1TP5 binding protein 1; PS1TP5BP1; anti-ACTB antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purification
Form/Format
PBS with 0.05% Proclin300, 50% glycerol, pH7.3.
Sequence Length
375
Applicable Applications for anti-ACTB antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:3000-1:30000
IHC: 1:100-1:300
IHC: 1:100-1:300
Immunogen
Recombinant Zebrafish Actin, cytoplasmic 2 protein
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded Rat heart using beta actin Polycloanl Antibody at dilution of 1:200)
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded Rat heart using beta actin Polycloanl Antibody at dilution of 1:200)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Rat small intestine using beta actin Polycloanl Antibody at dilution of 1:200)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Rat small intestine using beta actin Polycloanl Antibody at dilution of 1:200)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Rat small intestine using beta actin Polycloanl Antibody at dilution of 1:200)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Rat small intestine using beta actin Polycloanl Antibody at dilution of 1:200)
WB (Western Blot)
(Western Blot analysis of Raw264.7 cells using beta actin Polycloanl Antibody at dilution of 1:2000)
WB (Western Blot)
(Western Blot analysis of Raw264.7 cells using beta actin Polycloanl Antibody at dilution of 1:2000)
WB (Western Blot)
(Western Blot analysis of Rat brain and Mouse brain using beta actin Polycloanl Antibody at dilution of 1:2000)
WB (Western Blot)
(Western Blot analysis of Rat brain and Mouse brain using beta actin Polycloanl Antibody at dilution of 1:2000)
WB (Western Blot)
(Western Blot analysis of A431 cells using beta actin Polycloanl Antibody at dilution of 1:2000)
WB (Western Blot)
(Western Blot analysis of A431 cells using beta actin Polycloanl Antibody at dilution of 1:2000)
Related Product Information for anti-ACTB antibody
Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.
Product Categories/Family for anti-ACTB antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Calculated: 41 kDa
Observed: 41 kDa
Observed: 41 kDa
NCBI Official Full Name
actin, cytoplasmic 2
UniProt Protein Name
Actin, cytoplasmic 2
UniProt Gene Name
actbb
UniProt Synonym Gene Names
bactin2
Similar Products
Product Notes
The ACTB actbb (Catalog #AAA22143) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The beta ACTIN Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's beta ACTIN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:3000-1:30000 IHC: 1:100-1:300. Researchers should empirically determine the suitability of the ACTB actbb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta ACTIN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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