Application Data
(Published customer image: Rabbit anti beta glalctosidase antibody used for the demonstration of β-glalacosidase expressing cells in embryonic mouse tissues by immunofluorescence.Image caption:MB GABAergic pathway was severely compromised in the double and compound mutants. Expression of GABAergic-specific genes on the coronal sections of E12.5 in wild-type and single knockout embryos (A), as well as in compound and double knockout mutants (B). MB GABAergic induction occurred in double heterozygous mice based on the presence of GABAergic markers (Gad67 and Tal1). (A-E) The GABA phenotype observed at E12.5 in the vlMB of Mgn-/-Mash1-/- (--/--) mice (C2 and D3) was recovered in Mgn+/-Mash1+/- mice (D2 and E). The intermediate zone of m3 domain is magnified in E. GABAn of double heterozygous embryos were maintained at E18.5 (F) and until postnatal death P0 (G). DAPI, NKX2-2, TH, and beta-Gal staining were used as morphological markers). Arrows indicate the loss of postmitotic NKX2-2 neuronal subpopulation in the m2 domain, but not in the m4 domain (C). Scale bars: 500 um in A and G, and 50 um in E.From: Wende C-Z, Zoubaa S, Blak A, Echevarria D, Martinez S, Guillemot F, et al. (2015) Hairy/Enhancer-of-Split MEGANE and Proneural MASH1 Factors Cooperate Synergistically in Midbrain GABAergic Neurogenesis.PLoS ONE 10(5): e0127681.)
Rabbit anti-Bacterial BETA GALACTOSIDASE Polyclonal Antibody | anti-lacZ antibody
RABBIT ANTI BETA GALACTOSIDASE
Gene Names
lacZ; ECK0341; JW0335
Reactivity
Bacterial
Applications
Western Blot, ELISA, Immunohistochemistry
Synonyms
BETA GALACTOSIDASE, Antibody; RABBIT ANTI BETA GALACTOSIDASE; anti-lacZ antibody
Host
Rabbit
Reactivity
Bacterial
Clonality
Polyclonal
Isotype
IgG
Specificity
BETA GALACTOSIDASE
Form/Format
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
1024
Applicable Applications for anti-lacZ antibody
WB (Western Blot), ELISA, IHC (Immunohistochemistry)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Buffer Solution
Phosphate buffered saline
Immunogen
Beta-galactoside prepared from E.coli
Antiserum Preparation
Antisera to beta-galactosidase were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared from whole serum by ion exchange chromatography.
Preparation and Storage
Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch
Shelf Life: 18 months from date of despatch
Application Data
(Published customer image: Rabbit anti beta glalctosidase antibody used for the demonstration of β-glalacosidase expressing cells in embryonic mouse tissues by immunofluorescence.Image caption:MB GABAergic pathway was severely compromised in the double and compound mutants. Expression of GABAergic-specific genes on the coronal sections of E12.5 in wild-type and single knockout embryos (A), as well as in compound and double knockout mutants (B). MB GABAergic induction occurred in double heterozygous mice based on the presence of GABAergic markers (Gad67 and Tal1). (A-E) The GABA phenotype observed at E12.5 in the vlMB of Mgn-/-Mash1-/- (--/--) mice (C2 and D3) was recovered in Mgn+/-Mash1+/- mice (D2 and E). The intermediate zone of m3 domain is magnified in E. GABAn of double heterozygous embryos were maintained at E18.5 (F) and until postnatal death P0 (G). DAPI, NKX2-2, TH, and beta-Gal staining were used as morphological markers). Arrows indicate the loss of postmitotic NKX2-2 neuronal subpopulation in the m2 domain, but not in the m4 domain (C). Scale bars: 500 um in A and G, and 50 um in E.From: Wende C-Z, Zoubaa S, Blak A, Echevarria D, Martinez S, Guillemot F, et al. (2015) Hairy/Enhancer-of-Split MEGANE and Proneural MASH1 Factors Cooperate Synergistically in Midbrain GABAergic Neurogenesis.PLoS ONE 10(5): e0127681.)
Application Data
(Published customer image: Rabbit anti beta glalctosidase antibody used for the demonstration of β-glalacosidase expressing cells in embryonic mouse tissues by immunofluorescence.Image caption:MB GABAergic pathway was severely compromised in the double and compound mutants. Expression of GABAergic-specific genes on the coronal sections of E12.5 in wild-type and single knockout embryos (A), as well as in compound and double knockout mutants (B). MB GABAergic induction occurred in double heterozygous mice based on the presence of GABAergic markers (Gad67 and Tal1). (A-E) The GABA phenotype observed at E12.5 in the vlMB of Mgn-/-Mash1-/- (--/--) mice (C2 and D3) was recovered in Mgn+/-Mash1+/- mice (D2 and E). The intermediate zone of m3 domain is magnified in E. GABAn of double heterozygous embryos were maintained at E18.5 (F) and until postnatal death P0 (G). DAPI, NKX2-2, TH, and beta-Gal staining were used as morphological markers). Arrows indicate the loss of postmitotic NKX2-2 neuronal subpopulation in the m2 domain, but not in the m4 domain (C). Scale bars: 500 um in A and G, and 50 um in E.From: Wende C-Z, Zoubaa S, Blak A, Echevarria D, Martinez S, Guillemot F, et al. (2015) Hairy/Enhancer-of-Split MEGANE and Proneural MASH1 Factors Cooperate Synergistically in Midbrain GABAergic Neurogenesis.PLoS ONE 10(5): e0127681.)
Related Product Information for anti-lacZ antibody
Rabbit anti beta-galactosidase polyclonal antibody detects beta-galactosidase, an inducible enzyme that catalyzes the hydrolysis of lactose and other beta-galactosides into monosaccharides. Beta-galactosidase is a product of the LacZ operon, and is a homo-tetrameric protein consisting of four identical subunits of approximately 116 kDa each. beta-galactosidase is commonly used as a reporter gene in molecular biology to assess the efficiency of transfection.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Beta-galactosidase
NCBI Official Symbol
lacZ
NCBI Official Synonym Symbols
ECK0341; JW0335
NCBI Protein Information
beta-D-galactosidase
UniProt Protein Name
Beta-galactosidase
UniProt Gene Name
lacZ
UniProt Synonym Gene Names
Beta-gal
UniProt Entry Name
BGAL_ECOLI
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Product Notes
The lacZ lacz (Catalog #AAA50280) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RABBIT ANTI BETA GALACTOSIDASE reacts with Bacterial and may cross-react with other species as described in the data sheet. AAA Biotech's BETA GALACTOSIDASE can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA, IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the lacZ lacz for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BETA GALACTOSIDASE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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