FCM/FACS (Flow Cytometry)
(Blank control(blue):Hepg2 cells (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti- Biliverdin Reductase antibody(bs-12868R), Dilution: 1 ug in 100 uL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.)
Rabbit anti-Rat, Pig Biliverdin Reductase Polyclonal Antibody | anti-BLVRA antibody
Biliverdin Reductase Polyclonal Antibody
Gene Names
BLVRA; BVR; BLVR; BVRA
Reactivity
Rat, Pig
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Biliverdin Reductase, Antibody; Biliverdin Reductase Polyclonal Antibody; Biliverdin reductase A; BVR A; 1.3.1.24; Biliverdin-IX alpha-reductase; BLVRA; BLVR; BVR; anti-BLVRA antibody
Host
Rabbit
Reactivity
Rat, Pig
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Applicable Applications for anti-BLVRA antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Blank control(blue):Hepg2 cells (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti- Biliverdin Reductase antibody(bs-12868R), Dilution: 1 ug in 100 uL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.)
FCM/FACS (Flow Cytometry)
(Blank control(blue):Hepg2 cells (fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody:Rabbit Anti- Biliverdin Reductase antibody(bs-12868R), Dilution: 1 ug in 100 uL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.)
IHC (Immunohistochemistry)
(Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (Biliverdin) Polyclonal Antibody, Unconjugated (bs-12868R) at 1:500 overnight at 4 degree C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.)
IHC (Immunohistochemistry)
(Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (Biliverdin) Polyclonal Antibody, Unconjugated (bs-12868R) at 1:500 overnight at 4 degree C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
33 kDa
NCBI Official Full Name
Biliverdin reductase A
NCBI Official Synonym Full Names
biliverdin reductase A
NCBI Official Symbol
BLVRA
NCBI Official Synonym Symbols
BVR; BLVR; BVRA
NCBI Protein Information
biliverdin reductase A; BVR A; biliverdin-IX alpha-reductase
UniProt Protein Name
Biliverdin reductase A
UniProt Gene Name
BLVRA
UniProt Synonym Gene Names
BLVR; BVR; BVR A
UniProt Entry Name
BIEA_HUMAN
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Product Notes
The BLVRA blvra (Catalog #AAA291439) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Biliverdin Reductase Polyclonal Antibody reacts with Rat, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Biliverdin Reductase can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the BLVRA blvra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Biliverdin Reductase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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