Rabbit BLVRB Polyclonal Antibody | anti-BLVRB antibody

BLVRB Antibody (C-term)

Cat. Num. AAA290084

• Gene Names: BLVRB; FLR; BVRB; SDR43U1; HEL-S-10
• Reactivity: Human, mouse (Predicted Reactivity: Bovine)
• Applications: ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: BLVRB, Antibody; BLVRB Antibody (C-term); Flavin reductase (NADPH); FR; Biliverdin reductase B; BVR-B; Biliverdin-IX beta-reductase; Green heme-binding protein; GHBP; NADPH-dependent diaphorase; NADPH-flavin reductase; FLR; BLVRB; anti-BLVRB antibody

• Host: Rabbit
• Reactivity: Human, mouse (Predicted Reactivity: Bovine)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This BLVRB antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 161-175 amino acids from the C-terminal region of human BLVRB.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 206

• Applicable Applications for anti-BLVRB antibody: ELISA, WB (Western Blot)
• Function: Enzyme that can both act as a NAD(P)H-dependent reductase and a S-nitroso-CoA-dependent nitrosyltransferase (PubMed:10620517, PubMed:18241201, PubMed:27207795, PubMed:38056462, PubMed:7929092). Promotes fetal heme degradation during development (PubMed:10858451, PubMed:18241201, PubMed:7929092). Also expressed in adult tissues, where it acts as a regulator of hematopoiesis, intermediary metabolism (glutaminolysis, glycolysis, TCA cycle and pentose phosphate pathway) and insulin signaling (PubMed:27207795, PubMed:29500232, PubMed:38056462). Has a broad specificity oxidoreductase activity by catalyzing the NAD(P)H-dependent reduction of a variety of flavins, such as riboflavin, FAD or FMN, biliverdins, methemoglobin and PQQ (pyrroloquinoline quinone) (PubMed:10620517, PubMed:18241201, PubMed:7929092). Contributes to fetal heme catabolism by catalyzing reduction of biliverdin IXbeta into bilirubin IXbeta in the liver (PubMed:10858451, PubMed:18241201, PubMed:7929092). Biliverdin IXbeta, which constitutes the major heme catabolite in the fetus is not present in adult (PubMed:10858451, PubMed:18241201, PubMed:7929092). Does not reduce bilirubin IXalpha (PubMed:10858451, PubMed:18241201, PubMed:7929092). Can also reduce the complexed Fe(3+) iron to Fe(2+) in the presence of FMN and NADPH (PubMed:10620517). Acts as a protein nitrosyltransferase by catalyzing nitrosylation of cysteine residues of target proteins, such as HMOX2, INSR and IRS1 (PubMed:38056462). S- nitroso-CoA-dependent nitrosyltransferase activity is mediated via a 'ping-pong' mechanism: BLVRB first associates with both S-nitroso-CoA and protein substrate, nitric oxide group is then transferred from S- nitroso-CoA to Cys-109 and Cys-188 residues of BLVRB and from S- nitroso-BLVRB to the protein substrate (PubMed:38056462). Inhibits insulin signaling by mediating nitrosylation of INSR and IRS1, leading to their inhibition (PubMed:38056462).
• Cellular Location: Cytoplasm
• Tissue Location: Predominantly expressed in liver and erythrocytes (PubMed:7929092). At lower levels in heart, lung, adrenal gland and cerebrum (PubMed:7929092). Expressed in adult red blood cells (PubMed:29932944).
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks.; For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(All lanes : Anti-BLVRB Antibody (C-term) at 1:2000 dilutionLane 1: HepG2 whole cell lysateLane 2: human liver lysateLane 3: A549 whole cell lysateLane 4: U-251 MG whole cell lysateLane 5: mouse liver lysate Lane 6: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 22 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(Western blot analysis of lysates from human liver and mouse liver tissue lysate(from left to right), using BLVRB Antibody (C-term). AAA290084 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

Related Product Information for anti-BLVRB antibody

Broad specificity oxidoreductase that catalyzes the NADPH-dependent reduction of a variety of flavins, such as riboflavin, FAD or FMN, biliverdins, methemoglobin and PQQ (pyrroloquinoline quinone). Contributes to heme catabolism and metabolizes linear tetrapyrroles. Can also reduce the complexed Fe(3+) iron to Fe(2+) in the presence of FMN and NADPH. In the liver, converts biliverdin to bilirubin.

Product Categories/Family for anti-BLVRB antibody

Crown Antibodies; Metabolism; Signal Transduction

References

Chikuba K.,et al.Biochem. Biophys. Res. Commun. 198:1170-1176(1994).
Komuro A.,et al.Biol. Pharm. Bull. 19:796-804(1996).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Grimwood J.,et al.Nature 428:529-535(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 4502419
• NCBI GeneID: 645
• NCBI Accession #: NP_000704.1
• NCBI GenBank Nucleotide #: NM_000713.2
• UniProt Accession #: P30043; Q923D2; P52556
• Molecular Weight: 22119
• NCBI Official Full Name: flavin reductase (NADPH)
• NCBI Official Synonym Full Names: biliverdin reductase B
• NCBI Official Symbol: BLVRB
• NCBI Official Synonym Symbols: FLR; BVRB; SDR43U1; HEL-S-10
• NCBI Protein Information: flavin reductase (NADPH)
• UniProt Protein Name: Flavin reductase (NADPH)
• UniProt Gene Name: BLVRB
• UniProt Synonym Gene Names: FLR; FR; BVR-B; GHBP; FLR
• UniProt Entry Name: BLVRB_HUMAN