WB (Western Blot)
(Detection of human and mouse BMAL1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, MCF-7, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BMAL1 antibody AAA211942 (lot AAA211942-4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse BMAL1 Polyclonal Antibody | anti-BMAL1 antibody
Rabbit anti-BMAL1 Antibody, Affinity Purified
Gene Names
ARNTL; TIC; JAP3; MOP3; BMAL1; PASD3; BMAL1c; bHLHe5
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
BMAL1, Antibody; Rabbit anti-BMAL1 Antibody, Affinity Purified; class E basic helix-loop-helix protein 5; JAP3; testis tissue sperm-binding protein Li 50e; PASD3; PAS domain-containing protein 3; PAS domain containing 3; MOP3; member of PAS protein 3; TIC; basic helix-loop-helix family member e5; brain and muscle ARNT-like 1; member of PAS superfamily 3; aryl hydrocarbon receptor nuclear translocator-like protein 1; basic-helix-loop-helix-PAS orphan MOP3; basic-helix-loop-helix-PAS protein MOP3; bHLHe5; bHLH-PAS protein JAP3; BMAL1; BMAL1c; ARNT-like protein 1, brain and muscle; Aryl hydrocarbon receptor nuclear translocator-like protein 1; aryl hydrocarbon receptor nuclear translocator like; anti-BMAL1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-BMAL1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat, Chicken, Horse, Golden hamster, Orangutan
Immunogen
Between 576 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse BMAL1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, MCF-7, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BMAL1 antibody AAA211942 (lot AAA211942-4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse BMAL1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, MCF-7, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BMAL1 antibody AAA211942 (lot AAA211942-4) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human BMAL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BMAL1 antibody AAA211942 (lot AAA211942-4) used for IP at 6 ug per reaction. BMAL1 was also immunoprecipitated by a previous lot of this antibody (lot AAA211942-3). For blotting immunoprecipitated BMAL1, AAA211942 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human BMAL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BMAL1 antibody AAA211942 (lot AAA211942-4) used for IP at 6 ug per reaction. BMAL1 was also immunoprecipitated by a previous lot of this antibody (lot AAA211942-3). For blotting immunoprecipitated BMAL1, AAA211942 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Related Product Information for anti-BMAL1 antibody
BMAL1 [brain and muscle aryl hydrocarbon receptor nuclear translocator (ARNT)-like 1] is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This complex binds an E-box upstream of the PER1 gene, activating this gene and possibly other circadian rhythym-associated genes [taken from NCBI Entrez Gene (GeneID: 406)].
Product Categories/Family for anti-BMAL1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
626
NCBI Official Full Name
aryl hydrocarbon receptor nuclear translocator-like protein 1 isoform a
NCBI Official Synonym Full Names
aryl hydrocarbon receptor nuclear translocator-like
NCBI Official Symbol
ARNTL
NCBI Official Synonym Symbols
TIC; JAP3; MOP3; BMAL1; PASD3; BMAL1c; bHLHe5
NCBI Protein Information
aryl hydrocarbon receptor nuclear translocator-like protein 1; bHLH-PAS protein JAP3; member of PAS protein 3; member of PAS superfamily 3; brain and muscle ARNT-like 1; PAS domain-containing protein 3; ARNT-like protein 1, brain and muscle; basic-helix-l
UniProt Protein Name
Aryl hydrocarbon receptor nuclear translocator-like protein 1
UniProt Gene Name
ARNTL
UniProt Synonym Gene Names
BHLHE5; BMAL1; MOP3; PASD3; bHLHe5
UniProt Entry Name
BMAL1_HUMAN
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Product Notes
The BMAL1 arntl (Catalog #AAA211942) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-BMAL1 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BMAL1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the BMAL1 arntl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BMAL1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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