IP (Immunoprecipitation)
(Detection of human BORG4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BORG4 antibody AAA211861 used for IP at 10 ug/mg lysate. BORG4 was also immunoprecipitated by rabbit anti-BORG4 antibody which recognizes an upstream epitope. For blotting immunoprecipitated BORG4, AAA211861 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human BORG4 Polyclonal Antibody | anti-BORG4 antibody
Rabbit anti-BORG4 Antibody, Affinity Purified
Gene Names
CDC42EP4; CEP4; BORG4; KAIA1777
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
BORG4, Antibody; Rabbit anti-BORG4 Antibody, Affinity Purified; cdc42 effector protein 4; KAIA1777; CEP4; BORG4; binder of Rho GTPases 4; CDC42 effector protein (Rho GTPase binding) 4; Cdc42 effector protein 4; CDC42 effector protein 4; anti-BORG4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-BORG4 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen
Between 250 and 300
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human BORG4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BORG4 antibody AAA211861 used for IP at 10 ug/mg lysate. BORG4 was also immunoprecipitated by rabbit anti-BORG4 antibody which recognizes an upstream epitope. For blotting immunoprecipitated BORG4, AAA211861 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human BORG4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BORG4 antibody AAA211861 used for IP at 10 ug/mg lysate. BORG4 was also immunoprecipitated by rabbit anti-BORG4 antibody which recognizes an upstream epitope. For blotting immunoprecipitated BORG4, AAA211861 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human BORG4 by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-BORG4 (Cat. No. AAA211861 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human BORG4 by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-BORG4 (Cat. No. AAA211861 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-BORG4 antibody
BORG4 (binder of Rho GTPases 4) is part of a family of proteins that function as negative regulators of CDC42 Rho GTPase signaling. The Borg family of proteins are also known as CEPs (Cdc effector proteins) and have been shown to act downstream of Cdc42 to influence the assembly of actin filaments and changes in cell shape.
Product Categories/Family for anti-BORG4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,980 Da
NCBI Official Full Name
cdc42 effector protein 4
NCBI Official Synonym Full Names
CDC42 effector protein (Rho GTPase binding) 4
NCBI Official Symbol
CDC42EP4
NCBI Official Synonym Symbols
CEP4; BORG4; KAIA1777
NCBI Protein Information
cdc42 effector protein 4; binder of Rho GTPases 4
UniProt Protein Name
Cdc42 effector protein 4
UniProt Gene Name
CDC42EP4
UniProt Synonym Gene Names
BORG4; CEP4
UniProt Entry Name
BORG4_HUMAN
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Product Notes
The BORG4 cdc42ep4 (Catalog #AAA211861) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-BORG4 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BORG4 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the BORG4 cdc42ep4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BORG4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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