IHC (Immunohiostchemistry)
(The image on the left is immunohistochemistry of paraffin-embedded human tonsil tissue using 31224 (BRAF Antibody) at dilution 1/60, on the right is treated with the synthetic peptide.)
Rabbit anti-Human, Mouse BRAF Polyclonal Antibody | anti-BRAF antibody
BRAF Antibody
Gene Names
BRAF; NS7; BRAF1; RAFB1; B-RAF1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot, ELISA
Synonyms
BRAF, Antibody; BRAF Antibody; V-raf murine sarcoma viral oncogene homolog B1, NS7, BRAF1, RAFB1, B-RAF1; anti-BRAF antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of total BRAF protein.
Form/Format
Supplied at 2.1mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, 0.05% sodium azide and 50% glycerol.
Sequence Length
766
Applicable Applications for anti-BRAF antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen Type
Peptide
Immunogen Description
Synthetic peptide corresponding to a region derived from 591-607 amino acids of Human v-raf murine sarcoma viral oncogene homolog B1
Target Name
BRAF
Preparation and Storage
Store at -20 degree C/1 year
IHC (Immunohiostchemistry)
(The image on the left is immunohistochemistry of paraffin-embedded human tonsil tissue using 31224 (BRAF Antibody) at dilution 1/60, on the right is treated with the synthetic peptide.)
IHC (Immunohiostchemistry)
(The image on the left is immunohistochemistry of paraffin-embedded human tonsil tissue using 31224 (BRAF Antibody) at dilution 1/60, on the right is treated with the synthetic peptide.)
Application Data
(Gel: 8%SDS-PAGELane1: Hela cell lysateLane2: HT-29 cell lysateLysates: 50 ug per lanePrimary antibody: 1/1400 dilutionSecondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilutionExposure time: 5 minutes)
Application Data
(Gel: 8%SDS-PAGELane1: Hela cell lysateLane2: HT-29 cell lysateLysates: 50 ug per lanePrimary antibody: 1/1400 dilutionSecondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilutionExposure time: 5 minutes)
Related Product Information for anti-BRAF antibody
This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene.
Product Categories/Family for anti-BRAF antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
84kD
NCBI Official Full Name
serine/threonine-protein kinase B-raf
NCBI Official Synonym Full Names
B-Raf proto-oncogene, serine/threonine kinase
NCBI Official Symbol
BRAF
NCBI Official Synonym Symbols
NS7; BRAF1; RAFB1; B-RAF1
NCBI Protein Information
serine/threonine-protein kinase B-raf
UniProt Protein Name
Serine/threonine-protein kinase B-raf
UniProt Gene Name
BRAF
UniProt Synonym Gene Names
BRAF1; RAFB1
UniProt Entry Name
BRAF_HUMAN
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Product Notes
The BRAF braf (Catalog #AAA305168) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BRAF Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BRAF can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the BRAF braf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRAF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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