WB (Western Blot)
(Detection of human phospho-BRCA1 (S1280) by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells untreated (-) or treated (+) with Hydroxyurea. Antibody: Affinity purified rabbit anti-phospho-BRCA1 (S1280) antibody (AAA210718 lot 2) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human BRCA1 Polyclonal Antibody | anti-BRCA1 antibody
Rabbit anti-Phospho BRCA1 (S1280) Antibody, Affinity Purified
Gene Names
BRCA1; IRIS; PSCP; BRCAI; BRCC1; PNCA4; RNF53; BROVCA1; PPP1R53
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
BRCA1, Antibody; Rabbit anti-Phospho BRCA1 (S1280) Antibody, Affinity Purified; BRCA1, Phospho (S1280); IRIS; RING-type E3 ubiquitin transferase BRCA1; BRCAI; RING finger protein 53; RNF53; PSCP; protein phosphatase 1, regulatory subunit 53; PPP1R53; PNCA4; BRCA1/BRCA2-containing complex, subunit 1; breast and ovarian cancer susceptibility protein 1; BRCC1; breast cancer 1, early onset; breast cancer type 1 susceptibility protein; BROVCA1; early onset breast cancer 1; Fanconi anemia, complementation group S; FANCS; Breast cancer type 1 susceptibility protein; BRCA1, DNA repair associated; anti-BRCA1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-BRCA1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Monkey, Chimpanzee
Immunogen
Surrounding serine 1280
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human phospho-BRCA1 (S1280) by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells untreated (-) or treated (+) with Hydroxyurea. Antibody: Affinity purified rabbit anti-phospho-BRCA1 (S1280) antibody (AAA210718 lot 2) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human phospho-BRCA1 (S1280) by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells untreated (-) or treated (+) with Hydroxyurea. Antibody: Affinity purified rabbit anti-phospho-BRCA1 (S1280) antibody (AAA210718 lot 2) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human phospho-BRCA1 (S1280) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with Hydroxyurea. Antibodies: Affinity purified rabbit anti-phospho-BRCA1 (S1280) antibody (AAA210718 lot 2) used for IP at 6 ug per reaction. Phospho-BRCA1 (S1280) was also immunoprecipitated by a second phospho-BRCA1 antibody . For blotting immunoprecipitated phospho-BRCA1 (S1280), AAA210718 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human phospho-BRCA1 (S1280) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with Hydroxyurea. Antibodies: Affinity purified rabbit anti-phospho-BRCA1 (S1280) antibody (AAA210718 lot 2) used for IP at 6 ug per reaction. Phospho-BRCA1 (S1280) was also immunoprecipitated by a second phospho-BRCA1 antibody . For blotting immunoprecipitated phospho-BRCA1 (S1280), AAA210718 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-BRCA1 antibody
BRCA1 was identified as a gene with germline mutations that predisposes women to breast and ovarian cancer. The BRCA1 tumor suppressor is a large protein that has been demonstrated to interact with a multitude of proteins involved in the DNA damage response and cell cycle checkpoints to maintain genomic integrity. BRCA1 functions by regulating the activities of its interacting proteins which mediate processes such as gene transcription, gene repression, protein phosphorylation, and ubiquitination.
Product Categories/Family for anti-BRCA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
207,721 Da
NCBI Official Full Name
breast cancer type 1 susceptibility protein isoform 1
NCBI Official Synonym Full Names
breast cancer 1, early onset
NCBI Official Symbol
BRCA1
NCBI Official Synonym Symbols
IRIS; PSCP; BRCAI; BRCC1; PNCA4; RNF53; BROVCA1; PPP1R53
NCBI Protein Information
breast cancer type 1 susceptibility protein; RING finger protein 53; BRCA1/BRCA2-containing complex, subunit 1; protein phosphatase 1, regulatory subunit 53; breast and ovarian cancer susceptibility protein 1; breast and ovarian cancer sususceptibility pr
UniProt Protein Name
Breast cancer type 1 susceptibility protein
UniProt Gene Name
BRCA1
UniProt Synonym Gene Names
RNF53
UniProt Entry Name
BRCA1_HUMAN
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Product Notes
The BRCA1 brca1 (Catalog #AAA210718) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Phospho BRCA1 (S1280) Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BRCA1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the BRCA1 brca1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRCA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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