WB (Western Blot)
(Detection of human and mouse BRCC36 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-BRCC36 antibody AAA211908 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. BRCC36 was also immunoprecipitated by rabbit anti-BRCC36 antibody BL9195, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 second (A and B).)
Rabbit anti-Human, Mouse BRCC36 Polyclonal Antibody | anti-BRCC36 antibody
Rabbit anti-BRCC36 Antibody, Affinity Purified
Gene Names
BRCC3; C6.1A; BRCC36; CXorf53
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
BRCC36, Antibody; Rabbit anti-BRCC36 Antibody, Affinity Purified; C6.1A; BRCA1/BRCA2-containing complex subunit 36; lys-63-specific deubiquitinase BRCC36; CXorf53; BRCA1/BRCA2-containing complex subunit 3; BRCA1-A complex subunit BRCC36; BRCC36; BRISC complex subunit BRCC36; Lys-63-specific deubiquitinase BRCC36; anti-BRCC36 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-BRCC36 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 266 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse BRCC36 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-BRCC36 antibody AAA211908 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. BRCC36 was also immunoprecipitated by rabbit anti-BRCC36 antibody BL9195, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 second (A and B).)
WB (Western Blot)
(Detection of human and mouse BRCC36 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-BRCC36 antibody AAA211908 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. BRCC36 was also immunoprecipitated by rabbit anti-BRCC36 antibody BL9195, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 second (A and B).)
IHC (Immunohistochemistry)
(Detection of human BRCC36 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BRCC36 (Cat. No. AAA211908) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human BRCC36 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BRCC36 (Cat. No. AAA211908) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-BRCC36 antibody
BRCA1/BRCA2-containing complex subunit 36 (BRCC36) is a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. BRCC36 is a component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites and leads to targeting of the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, BRCC36 specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P46736].
Product Categories/Family for anti-BRCC36 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28,027 Da
NCBI Official Full Name
lys-63-specific deubiquitinase BRCC36 isoform 1
NCBI Official Synonym Full Names
BRCA1/BRCA2-containing complex, subunit 3
NCBI Official Symbol
BRCC3
NCBI Official Synonym Symbols
C6.1A; BRCC36; CXorf53
NCBI Protein Information
lys-63-specific deubiquitinase BRCC36; BRISC complex subunit BRCC36; BRCA1-A complex subunit BRCC36; BRCA1/BRCA2-containing complex subunit 36
UniProt Protein Name
Lys-63-specific deubiquitinase BRCC36
UniProt Gene Name
BRCC3
UniProt Synonym Gene Names
BRCC36; C6.1A; CXorf53
UniProt Entry Name
BRCC3_HUMAN
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Product Notes
The BRCC36 brcc3 (Catalog #AAA211908) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-BRCC36 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's BRCC36 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the BRCC36 brcc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRCC36, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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