Rabbit BRDT Polyclonal Antibody | anti-BRDT antibody

BRDT Antibody (N-term)

Cat. Num. AAA285696

• Gene Names: BRDT; CT9; BRD6
• Reactivity: Human (Predicted Reactivity: Monkey)
• Applications: ELISA, Western Blot
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: BRDT, Antibody; BRDT Antibody (N-term); Bromodomain testis-specific protein; Cancer/testis antigen 9; CT9; RING3-like protein; BRDT; anti-BRDT antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Monkey)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Clone Number: RB8492
• Specificity: This BRDT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human BRDT.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 1-30
• Sequence Length: 947

• Applicable Applications for anti-BRDT antibody: ELISA, WB (Western Blot)
• Function: Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis (PubMed: , PubMed: ). Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time (PubMed: ). In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA (PubMed: 22901802). Also recognizes and binds a subset of butyrylated histones: able to bind histone H4 butyrylated at 'Lys-8' (H4K8ac), while it is not able to bind H4 butyrylated at 'Lys-5' (H4K5ac) (By similarity). Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids (By similarity). Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin.
• Cellular Location: Nucleus Note=Detected on chromatin. {ECO:0000250|UniProtKB:Q91Y44}
• Tissue Location: Tissue Location
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(All lanes : Anti-BRDT N-term at 1:2000 dilutionLane 1: Human testis lysateLane 2: PC-3 whole cell lysateLane 3: MDA-MB-453 whole cell lysateLane 4: NCI-H1299 whole cell lysateLysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 108 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-BRDT antibody

BRDT is similar to the RING3 protein family. It possesses 2 bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain is found in proteins that regulate transcription. Two transcript variants encoding the same protein have been found for this gene. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 both encode the same protein.

References

Pivot-Pajot, C., et al., Mol. Cell. Biol. 23(15):5354-5365 (2003).
Dhalluin, C., et al., Nature 399(6735):491-496 (1999).
Jones, M.H., et al., Genomics 45(3):529-534 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 338797755
• NCBI GeneID: 676
• NCBI Accession #: NP_001229734.1; NP_001229735.1; NP_001229736.1; NP_001229737.1; NP_001229739.1; NP_001717.2; NP_997072.1
• NCBI GenBank Nucleotide #: NM_001242805.1
• UniProt Accession #: Q58F21; O14789; Q05DQ4; Q6P5T1; Q7Z4A6; Q8IWI6; A6NF68; B7Z811; B7Z890; B7ZAX7; D3DT32
• NCBI Official Full Name: bromodomain testis-specific protein isoform b
• NCBI Official Synonym Full Names: bromodomain, testis-specific
• NCBI Official Symbol: BRDT
• NCBI Official Synonym Symbols: CT9; BRD6
• NCBI Protein Information: bromodomain testis-specific protein
• UniProt Protein Name: Bromodomain testis-specific protein
• UniProt Gene Name: BRDT
• UniProt Synonym Gene Names: CT9
• UniProt Entry Name: BRDT_HUMAN