IP (Immunoprecipitation)
(Detection of human BTBD12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BTBD12 antibody AAA211825 used for IP at 10 ug/mg lysate. BTBD12 was also immunoprecipitated by rabbit anti-BTBD12 antibody which recognizes an upstream epitope. For blotting immunoprecipitated BTBD12, AAA211825 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human BTBD12 Polyclonal Antibody | anti-BTBD12 antibody
Rabbit anti-BTBD12 Antibody, Affinity Purified
Gene Names
SLX4; FANCP; BTBD12; MUS312
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
BTBD12, Antibody; Rabbit anti-BTBD12 Antibody, Affinity Purified; BTB/POZ domain-containing protein 12; BTBD12; FANCP; MUS312; structure-specific endonuclease subunit SLX4; Structure-specific endonuclease subunit SLX4; SLX4 structure-specific endonuclease subunit; anti-BTBD12 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-BTBD12 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen
Between 1784 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human BTBD12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BTBD12 antibody AAA211825 used for IP at 10 ug/mg lysate. BTBD12 was also immunoprecipitated by rabbit anti-BTBD12 antibody which recognizes an upstream epitope. For blotting immunoprecipitated BTBD12, AAA211825 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human BTBD12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BTBD12 antibody AAA211825 used for IP at 10 ug/mg lysate. BTBD12 was also immunoprecipitated by rabbit anti-BTBD12 antibody which recognizes an upstream epitope. For blotting immunoprecipitated BTBD12, AAA211825 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human BTBD12 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BTBD12 (Cat. No. AAA211825) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human BTBD12 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-BTBD12 (Cat. No. AAA211825) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-BTBD12 antibody
BTBD12 (BTBD12 domain-containing protein 12) has been identified as the human ortholog of the yeast Slx4p and drosophila MUS312 DNA repair factors. BTBD12 is an ATM/ATR checkpoint substrate that functions as part of a Holliday junction resolvase and is important for DNA interstrand crosslink repair.
Product Categories/Family for anti-BTBD12 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
200,012 Da
NCBI Official Full Name
structure-specific endonuclease subunit SLX4
NCBI Official Synonym Full Names
SLX4 structure-specific endonuclease subunit
NCBI Official Symbol
SLX4
NCBI Official Synonym Symbols
FANCP; BTBD12; MUS312
NCBI Protein Information
structure-specific endonuclease subunit SLX4; BTB (POZ) domain containing 12; BTB/POZ domain-containing protein 12; SLX4 structure-specific endonuclease subunit homolog
UniProt Protein Name
Structure-specific endonuclease subunit SLX4
UniProt Gene Name
SLX4
UniProt Synonym Gene Names
BTBD12; KIAA1784; KIAA1987
UniProt Entry Name
SLX4_HUMAN
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Product Notes
The BTBD12 slx4 (Catalog #AAA211825) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-BTBD12 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BTBD12 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the BTBD12 slx4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BTBD12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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