IP (Immunoprecipitation)
(Detection of human BTF3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BTF3 antibody AAA211843 used for IP at 10 ug/mg lysate. BTF3 was also immunoprecipitated by rabbit anti-BTF3 antibody BL5564, which recognizes an upstream epitope. For blotting immunoprecipitated BTF3, BL5564 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human BTF3 Polyclonal Antibody | anti-BTF3 antibody
Rabbit anti-BTF3 Antibody, Affinity Purified
Gene Names
BTF3; NACB; BTF3a; BTF3b; BETA-NAC
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
BTF3, Antibody; Rabbit anti-BTF3 Antibody, Affinity Purified; NAC-beta; transcription factor BTF3; RNA polymerase B transcription factor 3; nascent polypeptide-associated complex subunit beta; BETA-NAC; NACB; nascent-polypeptide-associated complex beta polypeptide; BTF3b; BTF3a; Transcription factor BTF3; basic transcription factor 3; anti-BTF3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-BTF3 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human BTF3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BTF3 antibody AAA211843 used for IP at 10 ug/mg lysate. BTF3 was also immunoprecipitated by rabbit anti-BTF3 antibody BL5564, which recognizes an upstream epitope. For blotting immunoprecipitated BTF3, BL5564 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human BTF3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BTF3 antibody AAA211843 used for IP at 10 ug/mg lysate. BTF3 was also immunoprecipitated by rabbit anti-BTF3 antibody BL5564, which recognizes an upstream epitope. For blotting immunoprecipitated BTF3, BL5564 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry)
(Detection of human BTF3 by immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and human prostate carcinoma (right). Antibody: Affinity purified rabbit anti-BTF3 (Cat. No. AAA211843 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human BTF3 by immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and human prostate carcinoma (right). Antibody: Affinity purified rabbit anti-BTF3 (Cat. No. AAA211843 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-BTF3 antibody
Basic transcription factor 3 (BTF3) is a general transcription factor that forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P20290].
Product Categories/Family for anti-BTF3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,699 Da
NCBI Official Full Name
transcription factor BTF3 isoform A
NCBI Official Synonym Full Names
basic transcription factor 3
NCBI Official Symbol
BTF3
NCBI Official Synonym Symbols
NACB; BTF3a; BTF3b; BETA-NAC
NCBI Protein Information
transcription factor BTF3; NAC-beta; RNA polymerase B transcription factor 3; nascent polypeptide-associated complex subunit beta; nascent-polypeptide-associated complex beta polypeptide
UniProt Protein Name
Transcription factor BTF3
UniProt Gene Name
BTF3
UniProt Synonym Gene Names
NACB; NAC-beta
UniProt Entry Name
BTF3_HUMAN
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Product Notes
The BTF3 btf3 (Catalog #AAA211843) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-BTF3 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BTF3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the BTF3 btf3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BTF3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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