IHC (Immunohiostchemistry)
(Dilution: WB: (1/500 - 1/1000), IH (1/100 - 1/200)Immunohistochemical analysis of C/EBP beta staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Rabbit C/EBP beta Polyclonal Antibody | anti-C/EBPbeta antibody
Anti-C/EBP beta Antibody
Gene Names
CEBPB; LAP; CRP2; TCF5; IL6DBP; NF-IL6; C/EBP-beta
Reactivity
Human, Bovine, Dog
Applications
Immunohistochemistry, Western Blot
Synonyms
C/EBP beta, Antibody; Anti-C/EBP beta Antibody; TCF5; CCAAT/enhancer-binding protein beta; C/EBP beta; Liver activator protein; LAP; Liver-enriched inhibitory protein; LIP; Nuclear factor NF-IL6; Transcription factor 5; TCF-5; anti-C/EBPbeta antibody
Host
Rabbit
Reactivity
Human, Bovine, Dog
Clonality
Polyclonal
Specificity
Recognizes endegous levels of C/EBP beta protein.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09 (W/V) sodium azide.
Applicable Applications for anti-C/EBPbeta antibody
IHC (Immunohistochemistry), WB (Western Blot)
Function
Important transcription factor regulating the expression of genes involved in immune and inflammatory responses (PubMed:12048245, PubMed:1741402, PubMed:18647749, PubMed:9374525). Also plays a significant role in adipogenesis, as well as in the gluconeogenic pathway, liver regeneration, and hematopoiesis. The consensus recognition site is 5'-T[TG]NNGNAA[TG]-3'. Its functional capacity is governed by protein interactions and post-translational protein modifications. During early embryogenesis, plays essential and redundant roles with CEBPA. Has a promitotic effect on many cell types such as hepatocytes and adipocytes but has an antiproliferative effect on T-cells by repressing MYC expression, facilitating differentiation along the T-helper 2 lineage. Binds to regulatory regions of several acute-phase and cytokines genes and plays a role in the regulation of acute-phase reaction and inflammation. Also plays a role in intracellular bacteria killing (By similarity). During adipogenesis, is rapidly expressed and, after activation by phosphorylation, induces CEBPA and PPARG, which turn on the series of adipocyte genes that give rise to the adipocyte phenotype. The delayed transactivation of the CEBPA and PPARG genes by CEBPB appears necessary to allow mitotic clonal expansion and thereby progression of terminal differentiation (PubMed:20829347). Essential for female reproduction because of a critical role in ovarian follicle development (By similarity). Restricts osteoclastogenesis: together with NFE2L1; represses expression of DSPP during odontoblast differentiation (By similarity).
Cellular location
Nucleus. Cytoplasm. Note=Translocates to the nucleus when phosphorylated at Ser-288. In T-cells when sumoylated drawn to pericentric heterochromatin thereby allowing proliferation (By similarity). {ECO:0000250|UniProtKB:P28033, ECO:0000269|PubMed:9374525}.
Tissue location
Expressed at low levels in the lung, kidney and spleen.
Preparation and Storage
Store at -20 degree. Stable for 12 months from date of receipt.
IHC (Immunohiostchemistry)
(Dilution: WB: (1/500 - 1/1000), IH (1/100 - 1/200)Immunohistochemical analysis of C/EBP beta staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
IHC (Immunohiostchemistry)
(Dilution: WB: (1/500 - 1/1000), IH (1/100 - 1/200)Immunohistochemical analysis of C/EBP beta staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
WB (Western Blot)
(Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)Western blot analysis of C/EBP beta expression in EC9706 (A), A549 (B) whole cell lysates.)
WB (Western Blot)
(Dilution: WB (1/500 - 1/1000), IH (1/100 - 1/200)Western blot analysis of C/EBP beta expression in EC9706 (A), A549 (B) whole cell lysates.)
Related Product Information for anti-C/EBPbeta antibody
Rabbit polyclonal antibody to C/EBP beta
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human C/EBP beta. The exact sequence is proprietary.
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human C/EBP beta. The exact sequence is proprietary.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated MW: 36106
NCBI Official Full Name
CCAAT/enhancer-binding protein beta
NCBI Official Synonym Full Names
CCAAT/enhancer binding protein (C/EBP), beta
NCBI Official Symbol
CEBPB
NCBI Official Synonym Symbols
LAP; CRP2; TCF5; IL6DBP; NF-IL6; C/EBP-beta
NCBI Protein Information
CCAAT/enhancer-binding protein beta; TCF-5; nuclear factor NF-IL6; transcription factor 5; nuclear factor of interleukin 6; interleukin 6-dependent DNA-binding protein; liver-enriched transcriptional activator protein
UniProt Protein Name
CCAAT/enhancer-binding protein beta
UniProt Gene Name
CEBPB
UniProt Synonym Gene Names
LAP; TCF5; C/EBP beta; TCF-5
UniProt Entry Name
CEBPB_HUMAN
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Product Notes
The C/EBPbeta cebpb (Catalog #AAA292258) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-C/EBP beta Antibody reacts with Human, Bovine, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's C/EBP beta can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the C/EBPbeta cebpb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C/EBP beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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