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WB (Western Blot)
(Western blot analysis of Phospho-C/EBP (ser181) in lysates of HuvEc , using Phospho-C/EBP (ser181) Antibody.)
Rabbit C/EBP epsilon Polyclonal Antibody | anti-C/EBP epsilon antibody
Phospho-C/EBP epsilon (ser181) Antibody
Gene Names
CEBPE; CRP1; C/EBP-epsilon
Reactivity
Human, Mouse, RatPredicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Synonyms
C/EBP epsilon, Antibody; Phospho-C/EBP epsilon (ser181) Antibody; C/EBP epsilon; CCAAT/enhancer binding protein (C/EBP) epsilon; CCAAT/enhancer binding protein epsilon; CCAAT/enhancer-binding protein epsilon; CEBPE; CEBPE_HUMAN; CRP 1; CRP1; anti-C/EBP epsilon antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Clonality
Polyclonal
Isotype
IgG
Specificity
Phospho-C/EBP-epsilon (ser181) Antibody detects endogenous levels of C/EBP-epsilon only when phosphorylated at ser181.
Purity/Purification
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
281
Applicable Applications for anti-C/EBP epsilon antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human C/EBP-epsilon around the phosphorylation site of ser181.
Tissue Specificity
Strongest expression occurs in promyelocyte and late-myeloblast-like cell lines.
Subcellular Location
Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of Phospho-C/EBP (ser181) in lysates of HuvEc , using Phospho-C/EBP (ser181) Antibody.)
WB (Western Blot)
(Western blot analysis of Phospho-C/EBP (ser181) in lysates of HuvEc , using Phospho-C/EBP (ser181) Antibody.)
IHC (Immunohistochemisry)
(At 1/100 staining human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemisry)
(At 1/100 staining human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohiostchemistry)
(At 1/100 staining rat heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohiostchemistry)
(At 1/100 staining rat heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemistry)
(At 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
IHC (Immunohistochemistry)
(At 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)
Related Product Information for anti-C/EBP epsilon antibody
Description: Transcriptional activator (PubMed:26019275). C/EBP are DNA-binding proteins that recognize two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers. Required for the promyelocyte-myelocyte transition in myeloid differentiation (PubMed:10359588).
Post Translational Modifications: Phosphorylated.
Subunit Structure: Binds DNA as a homodimer and as a heterodimer (PubMed:26019275). Can form stable heterodimers with CEBPA, CEBPB and CEBPD (By similarity). Interacts with GATA1 AND SPI1 (PubMed:26019275). Interacts with SMARCD2 (PubMed:28369036).
Similarity: Belongs to the bZIP family. C/EBP subfamily.
Post Translational Modifications: Phosphorylated.
Subunit Structure: Binds DNA as a homodimer and as a heterodimer (PubMed:26019275). Can form stable heterodimers with CEBPA, CEBPB and CEBPD (By similarity). Interacts with GATA1 AND SPI1 (PubMed:26019275). Interacts with SMARCD2 (PubMed:28369036).
Similarity: Belongs to the bZIP family. C/EBP subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 34 kDa
Predicted: 31 kDa
Predicted: 31 kDa
NCBI Official Full Name
CCAAT/enhancer-binding protein epsilon
NCBI Official Synonym Full Names
CCAAT enhancer binding protein epsilon
NCBI Official Symbol
CEBPE
NCBI Official Synonym Symbols
CRP1; C/EBP-epsilon
NCBI Protein Information
CCAAT/enhancer-binding protein epsilon
UniProt Protein Name
CCAAT/enhancer-binding protein epsilon
UniProt Gene Name
CEBPE
UniProt Synonym Gene Names
C/EBP epsilon
UniProt Entry Name
CEBPE_HUMAN
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Product Notes
The C/EBP epsilon cebpe (Catalog #AAA328522) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-C/EBP epsilon (ser181) Antibody reacts with Human, Mouse, Rat Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's C/EBP epsilon can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the C/EBP epsilon cebpe for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C/EBP epsilon, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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