WB (Western Blot)
(Detection of human c-Kit by western blot and immunoprecipitation. Samples: Whole cell lysate from human erythroleukemia (HEL) cells (50, 15, and 5 ug for WB; 1 mg for IP, 20% of IP loaded and HEK293T (T; 50 ug). Antibodies: Affinity purified rabbit anti-c-Kit antibody AAA212145 used for WB at 0.1 ug/ml (A) and 1.0 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).)
Rabbit anti-Human c-Kit Polyclonal Antibody | anti-c-Kit antibody
Rabbit anti-c-Kit Antibody, Affinity Purified
Gene Names
KIT; PBT; SCFR; C-Kit; CD117
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
c-Kit, Antibody; Rabbit anti-c-Kit Antibody, Affinity Purified; piebald trait protein; proto-oncogene c-Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; tyrosine-protein kinase Kit; soluble KIT variant 1; proto-oncogene tyrosine-protein kinase Kit; p145 c-kit; CD antigen CD117; MASTC; PBT; mast/stem cell growth factor receptor Kit; c-Kit protooncogene; C-Kit; CD117; SCFR; Mast/stem cell growth factor receptor Kit; KIT proto-oncogene receptor tyrosine kinase; anti-c-Kit antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-c-Kit antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 926 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human c-Kit by western blot and immunoprecipitation. Samples: Whole cell lysate from human erythroleukemia (HEL) cells (50, 15, and 5 ug for WB; 1 mg for IP, 20% of IP loaded and HEK293T (T; 50 ug). Antibodies: Affinity purified rabbit anti-c-Kit antibody AAA212145 used for WB at 0.1 ug/ml (A) and 1.0 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).)
WB (Western Blot)
(Detection of human c-Kit by western blot and immunoprecipitation. Samples: Whole cell lysate from human erythroleukemia (HEL) cells (50, 15, and 5 ug for WB; 1 mg for IP, 20% of IP loaded and HEK293T (T; 50 ug). Antibodies: Affinity purified rabbit anti-c-Kit antibody AAA212145 used for WB at 0.1 ug/ml (A) and 1.0 ug/ml (B) and used for IP at 3 ug/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human c-Kit by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-c-Kit (Cat. No. AAA212145 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human c-Kit by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-c-Kit (Cat. No. AAA212145 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-c-Kit antibody
c-Kit is the receptor for stem cell factor (mast cell growth factor). It is a transmembrane receptor with tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of the c-Kit receptor and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P10721].
Product Categories/Family for anti-c-Kit antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
109,865 Da
NCBI Official Full Name
mast/stem cell growth factor receptor Kit isoform 1
NCBI Official Synonym Full Names
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
NCBI Official Symbol
KIT
NCBI Official Synonym Symbols
PBT; SCFR; C-Kit; CD117
NCBI Protein Information
mast/stem cell growth factor receptor Kit; p145 c-kit; proto-oncogene c-Kit; piebald trait protein; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-oncogene tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like
UniProt Protein Name
Mast/stem cell growth factor receptor Kit
UniProt Gene Name
KIT
UniProt Synonym Gene Names
SCFR; SCFR; PBT
UniProt Entry Name
KIT_HUMAN
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Product Notes
The c-Kit kit (Catalog #AAA212145) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-c-Kit Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's c-Kit can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the c-Kit kit for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-Kit, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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