Rabbit anti-Human C-RAF Polyclonal Antibody | anti-C-RAF antibody

C-RAF (Phospho-Ser642) Antibody

Cat. Num. AAA302015

• Gene Names: RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
• Reactivity: Human
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
• Synonyms: C-RAF, Antibody; C-RAF (Phospho-Ser642) Antibody; C-RAF; C-Raf; CRAF; RAF-1; anti-C-RAF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of C-RAF only when phosphorylated at serine 642.
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 648

• Applicable Applications for anti-C-RAF antibody: WB (Western Blot)
• Immunogen Type: Peptide-KLH
• Immunogen Description: Peptide sequence around phosphorylation site of Serine 642(T-T-S(p)-P-R) derived from Human C-RAF.
• Modification: Phospho-Ser642
• Target Name: C-RAF
• Purity Note: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Preparation and Storage: Store at -20 degree C/1 year

WB (Western Blot)

(Western blot analysis of extracts from HuvEc cells and K562 cells treated with PMA using C-RAF (Phospho-Ser642) Antibody.The lane on the right is treated with the antigen-specific peptide.)

Related Product Information for anti-C-RAF antibody

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2-antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation. Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation.Bonner T.I., Nucleic Acids Res. 14:1009-1015(1986).Ota T., Nat. Genet. 36:40-45(2004).Mural R.J., Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

Product Categories/Family for anti-C-RAF antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4506401
• NCBI GeneID: 5894
• NCBI Accession #: NP_002871.1
• NCBI GenBank Nucleotide #: NM_002880.3
• UniProt Accession #: P04049; Q15278; Q9UC20; B0LPH8; B2R5N3
• Molecular Weight: SDS-PAGE MW: 74kd
• NCBI Official Full Name: RAF proto-oncogene serine/threonine-protein kinase
• NCBI Official Synonym Full Names: Raf-1 proto-oncogene, serine/threonine kinase
• NCBI Official Symbol: RAF1
• NCBI Official Synonym Symbols: NS5; CRAF; Raf-1; c-Raf; CMD1NN
• NCBI Protein Information: RAF proto-oncogene serine/threonine-protein kinase
• UniProt Protein Name: RAF proto-oncogene serine/threonine-protein kinase
• UniProt Gene Name: RAF1
• UniProt Synonym Gene Names: RAF; cRaf
• UniProt Entry Name: RAF1_HUMAN