WB (Western Blot)
(Detection of human and mouse C14orf166 by western blot. Samples: Whole cell lysate (15 ug) from U2OS, Jurkat, A-549, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-C14orf166 antibody (AAA213430 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse C14orf166 Polyclonal Antibody | anti-C14orf166 antibody
Rabbit anti-C14orf166 Antibody, Affinity Purified
Gene Names
C14orf166; CLE; CLE7; CGI99; RLLM1; CGI-99; LCRP369
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
C14orf166, Antibody; Rabbit anti-C14orf166 Antibody, Affinity Purified; hCLE1; RNA transcription, translation and transport factor protein; UPF0568 protein C14orf166; RLLM1; RLL motif containing 1; LCRP369; hCLE; CLE7 homolog; CLE7; CLE; CGI-99; C14orf166; CGI99; RNA transcription, translation and transport factor; anti-C14orf166 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-C14orf166 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 75 and 125
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse C14orf166 by western blot. Samples: Whole cell lysate (15 ug) from U2OS, Jurkat, A-549, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-C14orf166 antibody (AAA213430 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse C14orf166 by western blot. Samples: Whole cell lysate (15 ug) from U2OS, Jurkat, A-549, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-C14orf166 antibody (AAA213430 lot 1) used for WB at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human C14orf166 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-C14orf166 antibody (AAA213430 lot 1) used for IP at 1 ug per reaction. C14orf166 was also immunoprecipitated by rabbit anti-C14orf166 antibody BL23425. For blotting immunoprecipitated C14orf166, AAA213430 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human C14orf166 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-C14orf166 antibody (AAA213430 lot 1) used for IP at 1 ug per reaction. C14orf166 was also immunoprecipitated by rabbit anti-C14orf166 antibody BL23425. For blotting immunoprecipitated C14orf166, AAA213430 was used at 0.04 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-C14orf166 antibody
C14orf166 is an RNA-binding protein involved in modulation of mRNA transcription by Polymerase II. Component of the tRNA-splicing ligase complex and is required for tRNA ligation. May be required for RNA transport. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9Y224].
Product Categories/Family for anti-C14orf166 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
28,068 Da
NCBI Official Full Name
UPF0568 protein C14orf166
NCBI Official Synonym Full Names
chromosome 14 open reading frame 166
NCBI Official Symbol
C14orf166
NCBI Official Synonym Symbols
CLE; CLE7; CGI99; RLLM1; CGI-99; LCRP369
NCBI Protein Information
UPF0568 protein C14orf166; CLE7 homolog; RLL motif containing 1
UniProt Protein Name
UPF0568 protein C14orf166
UniProt Gene Name
C14orf166
UniProt Synonym Gene Names
CGI-99; CLE
UniProt Entry Name
CN166_HUMAN
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Product Notes
The C14orf166 c14orf166 (Catalog #AAA213430) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-C14orf166 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's C14orf166 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the C14orf166 c14orf166 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C14orf166, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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