FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of RT4 cells using anti-C19orf2/URI1 antibody (AAA127280).Overlay histogram showing RT4 cells stained with AAA127280 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C19orf2/URI1 Antibody (AAA127280, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
Rabbit anti-Human, Mouse C19orf2/URI1 Polyclonal Antibody | anti-C19orf2/URI1 antibody
Anti-C19orf2/URI1 Antibody Picoband
Gene Names
URI1; RMP; URI; NNX3; C19orf2; PPP1R19
Reactivity
Human, Mouse
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Purity
Immunogen affinity purified.
Synonyms
C19orf2/URI1, Antibody; Anti-C19orf2/URI1 Antibody Picoband; Zinc finger protein Helios; IKAROS family zinc finger 2; Ikaros family zinc finger protein 2; IKZF2; HELIOS, ZNFN1A2; anti-C19orf2/URI1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Ubiquitous.High expression in liver, spleen, pancreas and kidney.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized. Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Applicable Applications for anti-C19orf2/URI1 antibody
ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
E coli-derived human C19orf2/URI1 recombinant protein (Position: K89-Q532).
Subcellular Localization
Nucleus
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500ug/ml.
Cross Reactivity
No cross-reactivity with other proteins.
Protein Function
Associates with Ikaros at centromeric heterochromatin.
Preparation and Storage
At -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months.Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of RT4 cells using anti-C19orf2/URI1 antibody (AAA127280).Overlay histogram showing RT4 cells stained with AAA127280 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C19orf2/URI1 Antibody (AAA127280, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of RT4 cells using anti-C19orf2/URI1 antibody (AAA127280).Overlay histogram showing RT4 cells stained with AAA127280 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-C19orf2/URI1 Antibody (AAA127280, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)
WB (Western Blot)
(Figure 1. Western blot analysis of C19orf2/URI1 using anti-C19orf2/URI1 antibody (AAA127280).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human U-87MG whole cell lysates,Lane 2: human K562 whole cell lysates,Lane 3: human Daudi whole cell lysates,Lane 4: human HEL1 whole cell lysates,Lane 5: mouse ovary tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C19orf2/URI1 antigen affinity purified polyclonal antibody (#AAA127280) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for C19orf2/URI1 at approximately 80 kDa. The expected band size for C19orf2/URI1 is at 60 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of C19orf2/URI1 using anti-C19orf2/URI1 antibody (AAA127280).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human U-87MG whole cell lysates,Lane 2: human K562 whole cell lysates,Lane 3: human Daudi whole cell lysates,Lane 4: human HEL1 whole cell lysates,Lane 5: mouse ovary tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C19orf2/URI1 antigen affinity purified polyclonal antibody (#AAA127280) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for C19orf2/URI1 at approximately 80 kDa. The expected band size for C19orf2/URI1 is at 60 kDa.)
Related Product Information for anti-C19orf2/URI1 antibody
The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Product Categories/Family for anti-C19orf2/URI1 antibody
References
1. Chaves-Perez, A., Yilmaz, M., Perna, C., de la Rosa, S., Djouder, N. URI is required to maintain intestinal architecture during ionizing radiation. Science 364: eaaq1165, 2019. Note: Electronic Article.2. Dorjsuren, D., Lin, Y., Wei, W., Yamashita, T., Nomura, T., Hayashi, N., Murakami, S. RMP, a novel RNA polymerase II subunit 5-interacting protein, counteracts transactivation by hepatitis B virus X protein. Molec. Cell Biol. 18: 7546-7555, 1998. 3. Gstaiger, M., Luke, B., Hess, D., Oakeley, E. J., Wirbelauer, C., Blondel, M., Vigneron, M., Peter, M., Krek, W. Control of nutrient-sensitive transcription programs by the unconventional prefoldin URI. Science 302: 1208-1212, 2003.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,447 Da
NCBI Official Full Name
unconventional prefoldin RPB5 interactor 1 isoform c
NCBI Official Synonym Full Names
URI1, prefoldin-like chaperone
NCBI Official Symbol
URI1
NCBI Official Synonym Symbols
RMP; URI; NNX3; C19orf2; PPP1R19
NCBI Protein Information
unconventional prefoldin RPB5 interactor 1; RPB5-mediating protein; protein phosphatase 1 regulatory subunit 19; protein phosphatase 1, regulatory subunit 19; RNA polymerase II subunit 5-mediating protein
UniProt Protein Name
Unconventional prefoldin RPB5 interactor 1
UniProt Gene Name
URI1
UniProt Synonym Gene Names
C19orf2; NNX3; PPP1R19; RMP; URI; RPB5-mediating protein
UniProt Entry Name
RMP_HUMAN
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Product Notes
The C19orf2/URI1 uri1 (Catalog #AAA127280) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-C19orf2/URI1 Antibody Picoband reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's C19orf2/URI1 can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the C19orf2/URI1 uri1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C19orf2/URI1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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