IP (Immunoprecipitation)
(C1orf109 was immunoprecipitated using:Lane A:0.5 mg U251MG Whole Cell Lysate4 uL anti-C1orf109 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-C1orf109 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size :26 kDa)
Rabbit anti-Human C1orf109 Polyclonal Antibody | anti-C1orf109 antibody
Anti-C1orf109 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
C1orf109, Antibody; Anti-C1orf109 Antibody, Rabbit Polyclonal; C1orf109 Antibody, Rabbit PAb, Antigen Affinity Purified; anti-C1orf109 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human C1orf109
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-C1orf109 antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived Human C1orf109 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human C1orf109 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(C1orf109 was immunoprecipitated using:Lane A:0.5 mg U251MG Whole Cell Lysate4 uL anti-C1orf109 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-C1orf109 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size :26 kDa)
IP (Immunoprecipitation)
(C1orf109 was immunoprecipitated using:Lane A:0.5 mg U251MG Whole Cell Lysate4 uL anti-C1orf109 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-C1orf109 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size :26 kDa)
IHC (Immunohiostchemistry)
(Immunochemical staining of human C1orf109 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human C1orf109 in human kidney with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
WB (Western Blot)
(Anti-C1orf109 rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLane B: RPMI-8226 Whole Cell LysateLane C: U251MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:23 kDaObserved band size:26 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-C1orf109 rabbit polyclonal antibody at 1:500 dilutionLane A: K562 Whole Cell LysateLane B: RPMI-8226 Whole Cell LysateLane C: U251MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:23 kDaObserved band size:26 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-C1orf109 antibody
Homo Sapiens Chromosome 1 ORF109 (c1orf109, GenBank ID: NM_017850.1), which encodes a substrate of CK2. C1orf109 gene is located on 1p34.3. The function of the gene has been poorly studied. Several research groups identified that C1orf109 dysregulation might involve in the developmental process or cause diseases such as tumors. Deletion of 1p34.3 locus, which includes C1orf109, could cause facial dysmorphism. The promoter region of C1orf109 gene was modified by methylation in aging and some diseases, such as keloids and systemic lupus erythematosus. C1ORF109 may be the downstream target of protein kinase CK2 and involved in the regulation of cancer cell proliferation.
References
Liu SS, et al. (2012) Identification and characterization of a novel gene, c1orf109, encoding a ck2 substrate that is involved in cancer cell proliferation. J Biomed Sci 19 (1): 49.Dou P, et al. (2020) C1orf109l binding dhx9 promotes DNA damage depended on the r-loop accumulation and enhances camptothecin chemosensitivity. Cell Prolif 53 (9): e12875.
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Product Notes
The C1orf109 (Catalog #AAA256679) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-C1orf109 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's C1orf109 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the C1orf109 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C1orf109, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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