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IF (Immunofluorescence)
(AAA328882 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328882 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Rabbit C3 Polyclonal Antibody | anti-C3 antibody
C3 Antibody
Gene Names
C3; ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, ELISA, Immunocytochemistry, Immunofluorescence
Purity
Peptide affinity purification
Synonyms
C3, Antibody; C3 Antibody; acylation-stimulating protein cleavage product; AHUS5; ARMD9; ASP; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3; c3 complement; C3a anaphylatoxin; C3a; C3b; CO3_HUMAN; Complement C3; Complement C3c alpha' chain fragment 2; Complement C3c; Complement component 3; Complement component C3; Complement component C3a; Complement component C3b; Complement factor 3; CPAMD1; Prepro C3; anti-C3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
C3 Antibody detects endogenous levels of total C3
Purity/Purification
Peptide affinity purification
Form/Format
IgG in phosphate buffered saline , pH 7.4, 150mMNaCl, 0.02% sodium azide and 50% glycero1
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-C3 antibody
IHC (Immunohistochemistry), ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence)
Immunogen
A synthesized peptide derived from human C3, corresponding to a region within the internal amino acids.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA328882 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328882 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IF (Immunofluorescence)
(AAA328882 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA328882 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IHC (Immunohistochemisry)
(AAA328882 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohistochemisry)
(AAA328882 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA328882 at 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(AAA328882 at 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of extracts from Mouse brain, using C3 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Mouse brain, using C3 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-C3 antibody
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
187kDa
NCBI Official Full Name
Complement component 3
NCBI Official Synonym Full Names
complement C3
NCBI Official Symbol
C3
NCBI Official Synonym Symbols
ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
NCBI Protein Information
complement C3
UniProt Protein Name
Complement C3
UniProt Gene Name
C3
UniProt Synonym Gene Names
CPAMD1; ASP
UniProt Entry Name
CO3_HUMAN
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Product Notes
The C3 c3 (Catalog #AAA328882) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The C3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's C3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the C3 c3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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