WB (Western Blot)
(Detection of human C3G by western blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-C3G antibody AAA211699 (lot AAA211699-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human C3G Polyclonal Antibody | anti-C3G antibody
Rabbit anti-C3G Antibody, Affinity Purified
Gene Names
RAPGEF1; C3G; GRF2
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
C3G, Antibody; Rabbit anti-C3G Antibody, Affinity Purified; GRF2; rap guanine nucleotide exchange factor 1; Rap guanine nucleotide exchange factor (GEF) 1; Protein C3G; Guanine nucleotide-releasing factor 2; CRK SH3-binding GNRP; C3G; guanine nucleotide-releasing factor 2 (specific for crk proto-oncogene); Rap guanine nucleotide exchange factor 1; anti-C3G antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-C3G antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 50 and 100
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human C3G by western blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-C3G antibody AAA211699 (lot AAA211699-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human C3G by western blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-C3G antibody AAA211699 (lot AAA211699-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human C3G by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-C3G antibody AAA211699 (lot AAA211699-2) used for IP at 6 ug per reaction. C3G was also immunoprecipitated by rabbit anti-C3G antibody For blotting immunoprecipitated C3G, AAA211699 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human C3G by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-C3G antibody AAA211699 (lot AAA211699-2) used for IP at 6 ug per reaction. C3G was also immunoprecipitated by rabbit anti-C3G antibody For blotting immunoprecipitated C3G, AAA211699 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-C3G antibody
CRK SH3-binding GNRP (C3G) is a guanine nucleotide exchange factor (GEF) that binds to the SH3 domain of CRK and GRB2/ASH and catalyzes the exchange of GDP for GTP on GTPases of the Ras family. C3G has been reported to be involved in signaling that mediates cell adhesion and migration.
Product Categories/Family for anti-C3G antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
120,548 Da
NCBI Official Full Name
rap guanine nucleotide exchange factor 1 isoform a
NCBI Official Synonym Full Names
Rap guanine nucleotide exchange factor (GEF) 1
NCBI Official Symbol
RAPGEF1
NCBI Official Synonym Symbols
C3G; GRF2
NCBI Protein Information
rap guanine nucleotide exchange factor 1; CRK SH3-binding GNRP; guanine nucleotide-releasing factor 2 (specific for crk proto-oncogene)
UniProt Protein Name
Rap guanine nucleotide exchange factor 1
UniProt Gene Name
RAPGEF1
UniProt Synonym Gene Names
GRF2
UniProt Entry Name
RPGF1_HUMAN
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Product Notes
The C3G rapgef1 (Catalog #AAA211699) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-C3G Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's C3G can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the C3G rapgef1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C3G, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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