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product-image-AAA287430_IF10.jpg IF (Immunofluorescence) (Confocal immunofluorescent analysis of C6 Antibody (N-term) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Rabbit anti-Human C6 Polyclonal Antibody | anti-C6 antibody

C6 Antibody (N-term)

Reactivity
Human
Applications
Western Blot, Immunofluorescence, Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
C6, Antibody; C6 Antibody (N-term); Complement component C6; C6; anti-C6 antibody
Ordering
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This C6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-58 amino acids from the N-terminal region of human C6.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.45 (varies by lot)
Sequence Positions
30-58
Sequence Length
934
Applicable Applications for anti-C6 antibody
WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IF (Immunofluorescence)

(Confocal immunofluorescent analysis of C6 Antibody (N-term) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

product-image-AAA287430_IF10.jpg IF (Immunofluorescence) (Confocal immunofluorescent analysis of C6 Antibody (N-term) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of MDA-231 cells using C6 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

product-image-AAA287430_FCM11.jpg FCM/FACS (Flow Cytometry) (Flow cytometric analysis of MDA-231 cells using C6 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with C6 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

product-image-AAA287430_IHC13.jpg IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human breast carcinoma reacted with C6 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of lysate from human blood plasma tissue lysate, using C6 Antibody (N-term). AAA287430 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)

product-image-AAA287430_WB15.jpg WB (Western Blot) (Western blot analysis of lysate from human blood plasma tissue lysate, using C6 Antibody (N-term). AAA287430 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
Related Product Information for anti-C6 antibody
C6 is a component of complement cascade. It is part of the membrane attack complex which can insert into the cell membrane and cause cell to lyse. People with C6 deficiency are prone to bacterial infection.
Product Categories/Family for anti-C6 antibody
References
Wu,C., et.al., Proteomics 7 (11), 1775-1785 (2007)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
729
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
104786
NCBI Official Full Name
complement component C6
NCBI Official Synonym Full Names
complement component 6
NCBI Official Symbol
C6
NCBI Protein Information
complement component C6
UniProt Protein Name
Complement component C6
UniProt Gene Name
C6
UniProt Entry Name
CO6_HUMAN

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Product Notes

The C6 c6 (Catalog #AAA287430) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 30-58. The C6 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's C6 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the C6 c6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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