IHC (Immunohistochemisry)
(Dilution: Tissue/cell: Human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-C6orf222 Polyclonal Antibody, Unconjugated(bs-15241R) 1:500, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)
Rabbit anti-Rat C6orf222 Polyclonal Antibody | anti-C6orf222 antibody
C6orf222 Polyclonal Antibody
Reactivity
Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
C6orf222, Antibody; C6orf222 Polyclonal Antibody; Protein BNIP5; BNIP5; C6orf222; anti-C6orf222 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Applicable Applications for anti-C6orf222 antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(Dilution: Tissue/cell: Human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-C6orf222 Polyclonal Antibody, Unconjugated(bs-15241R) 1:500, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)
IHC (Immunohistochemisry)
(Dilution: Tissue/cell: Human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-C6orf222 Polyclonal Antibody, Unconjugated(bs-15241R) 1:500, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)
IHC (Immunohiostchemistry)
(Tissue/cell: human liver cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-C6orf222 Polyclonal Antibody, Unconjugated(bs-15241R) 1:500, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)
IHC (Immunohiostchemistry)
(Tissue/cell: human liver cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37 degree C for 20 min;Incubation: Anti-C6orf222 Polyclonal Antibody, Unconjugated(bs-15241R) 1:500, overnight at 4 degree C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)
WB (Western Blot)
(Sample:U937 Cell (Human) Lysate at 30 ugLOVO Cell (Human) Lysate at 30 ugA549 Cell (Human) Lysate at 30 ugPrimary: Anti-C6orf222 (bs-15241R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 42 kDObserved band size: 42 kD)
WB (Western Blot)
(Sample:U937 Cell (Human) Lysate at 30 ugLOVO Cell (Human) Lysate at 30 ugA549 Cell (Human) Lysate at 30 ugPrimary: Anti-C6orf222 (bs-15241R) at 1/300 dilutionSecondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilutionPredicted band size: 42 kDObserved band size: 42 kD)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
71,930 Da
NCBI Official Full Name
uncharacterized protein C6orf222
NCBI Official Synonym Full Names
chromosome 6 open reading frame 222
NCBI Official Symbol
C6orf222
NCBI Protein Information
uncharacterized protein C6orf222
UniProt Protein Name
Uncharacterized protein C6orf222
UniProt Gene Name
C6orf222
UniProt Entry Name
CF222_HUMAN
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Product Notes
The C6orf222 c6orf222 (Catalog #AAA291609) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The C6orf222 Polyclonal Antibody reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's C6orf222 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the C6orf222 c6orf222 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C6orf222, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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