WB (Western Blot)
(Anti-CACNA2D2 Antibody (Center) (AAA290295) at 1:2000 dilution + mouse kidney lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 130 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human, Mouse CACNA2D2 Polyclonal Antibody | anti-CACNA2D2 antibody
CACNA2D2 Antibody (Center)
Gene Names
CACNA2D2; CACNA2D
Reactivity
Human, Mouse
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
CACNA2D2, Antibody; CACNA2D2 Antibody (Center); Voltage-dependent calcium channel subunit alpha-2/delta-2; Voltage-gated calcium channel subunit alpha-2/delta-2; Voltage-dependent calcium channel subunit alpha-2-2; Voltage-dependent calcium channel subunit delta-2; CACNA2D2; KIAA0558; anti-CACNA2D2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This CACNA2D2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 643-671 amino acids from the Central region of human CACNA2D2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
643-671
Sequence Length
1,152
Applicable Applications for anti-CACNA2D2 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Acts as a regulatory subunit for P/Q- type calcium channel (CACNA1A), N-type (CACNA1B), L-type (CACNA1C OR CACNA1D) and possibly T-type (CACNA1G) (PubMed:15111129, PubMed:23339110). Overexpression induces apoptosis.
Cellular Location
Membrane; Single-pass type I membrane protein. Note=Colocalizes with CACNA1A in lipid raft fractions.
Tissue Location
Predominantly present in cerebellar cortex. Present in various lung tumor cell lines, while it is absent in normal lung (at protein level). Highly expressed in heart, lung, testis, pancreas and skeletal muscle. Also expressed in kidney, liver, placenta and brain
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Anti-CACNA2D2 Antibody (Center) (AAA290295) at 1:2000 dilution + mouse kidney lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 130 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-CACNA2D2 Antibody (Center) (AAA290295) at 1:2000 dilution + mouse kidney lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 130 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-CACNA2D2 Antibody (Center) (AAA290295) at 1:2000 dilution + human testis lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 130 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-CACNA2D2 Antibody (Center) (AAA290295) at 1:2000 dilution + human testis lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 130 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
IHC (Immunohistochemistry)
(CACNA2D2 Antibody (Center) (AAA290295) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CACNA2D2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemistry)
(CACNA2D2 Antibody (Center) (AAA290295) immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CACNA2D2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-CACNA2D2 antibody
This gene encodes a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized.
Product Categories/Family for anti-CACNA2D2 antibody
References
Donato, R., et al. J. Neurosci. 26(48):12576-12586(2006) Ivanov, S.V., et al. Am. J. Pathol. 165(3):1007-1018(2004) Carboni, G.L., et al. Oncogene 22(4):615-626(2003) Honorio, S., et al. Mol. Cell. Probes 15(6):391-393(2001) Angeloni, D., et al. Mol. Cell. Probes 15(2):125-127(2001)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
voltage-dependent calcium channel subunit alpha-2/delta-2 isoform a
NCBI Official Synonym Full Names
calcium channel, voltage-dependent, alpha 2/delta subunit 2
NCBI Official Symbol
CACNA2D2
NCBI Official Synonym Symbols
CACNA2D
NCBI Protein Information
voltage-dependent calcium channel subunit alpha-2/delta-2
UniProt Protein Name
Voltage-dependent calcium channel subunit alpha-2/delta-2
UniProt Gene Name
CACNA2D2
UniProt Synonym Gene Names
KIAA0558
UniProt Entry Name
CA2D2_HUMAN
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Product Notes
The CACNA2D2 cacna2d2 (Catalog #AAA290295) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 643-671. The CACNA2D2 Antibody (Center) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CACNA2D2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CACNA2D2 cacna2d2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CACNA2D2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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